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TCC Nebauer Vocab 2

Conjugation Transfer of plasmid DNA from donor to recipient via a pilus
Obligate anaerobe Organisms for which oxygen is poison
Prophage Inactive virus within a cell that often incorporates it's DNA into the host chromosome
Lagging Strand Strand of DNA that is synthesized discontinuously in replication
Vector Nucleic acid molecules used to introduce foreign DNA or gene into a cell
Southern Blot Where DNA molecules are transferred to a nitrocellulose membrane for screening
Methylation Addition of methyl groups to one or two bases of a specific nucleotide squence
Halophile Organisms requiring high Salt environments
Mutagen Physical or chemical agents that may alter the normal DNA swquence
Mesophile Organisms that grow best at temperatures between 20 C and 40 C
Plaque Clear zone on a lawn of bacteria that indicates viral replication
R Factors Genes on plasmid's that provide antibiotic resistance
Recombinant DNA Contains homologous segments of DNA from two different genomes (or organisms)
Transformation A cell takes up naked DNA from the environment and incorporates it into it's genome
Recombination Exchange of homologous segments of DNA
Hfr Strain Where an F plasmid is integrated into the bacterial chromosome at a specific site
Gel Electrophoresis Method used to isolate fragments of DNA based on their size and charge
Reverse transcriptase Viral enzyme tha makes copies of DNA from RNA designated cDNA
Nucleotide analogs Compounds that are structurally similar to normal nucleotides
Reactive oxygen species Oxidizing agents that are poisonous to anaerobes
Frameshift mutation Insertion or deletion of nucleotide base pairs to cause a change in reading frame
F Factor Plasmid containing a gene for conjugation pilus
DNA Fingerprinting Method used to identify individuals by their unique DNA swquences
Polymerase chain reaction Technique used to produce large number of identical DNA molecules in vitro
Transduction Involves transfer of bacterial DNA from one cell to another via a replication virus
operon Consists of a promoter, and operator and a series of functional genes
Competence The ability to take up DNA from the environment and undergo transformation
Inclusion Bodies Signs of viral presence in infected cells see primarily in the host cell nucleus
Log Phase Period during which bacteria undergo rapid growth and reproduction
Thymine Dimer Adjacent thymine bases covalently bond to one another causing a mutation
Transposon DNA that moves form one location to another on the same or other chromosome
Ames test A fast, inexpensive method for screening mutagens
Represor protein Molecule that binds an operator to stop transcription of mRNA
Chemoautotroph Use organic compounds for energy and carbon dioxide as a carbon sourse
Facultative Orgnaisms that can survive either aerobically or anaerobically
Photoheterotroph Use light as an energy source and organic compounds as a carbon source
Gene probe Labeled nucleic acid molecules of specific
Bacteriophage A virus that infects bacteria
Promoter Location on an operon to which RNA poymerase binds.
Lysogenic cycle Replication cycle in which virally-infected host cells grow and reproduce before lysis
Created by: sarah.teacher36