Question | Answer |
Haemophilus influenzae
Morphology, Media, Test | GN coccobacillus
ONLY grows on CHOC
Req. X and Y to grow |
Haemophilus parainfluenzae
Morphology, Media, Test | GN coccobacillus
ONLY grows on CHOC
Only req. V to grow |
Haemophilus parahemolyticus
Morphology, Media, Test | GN coccbacillus
ONLY grows on CHOC
Only req. V to grow, Hem+ |
Haemophilus paraphrohiles
Morphology, Media, Test | GN coccbacillus
ONLY grow on CHOC
Only req. V to grow |
Staphylococcus aureus
Morphology, Media | GPC(clusters)
BAP=beta hem
MSA=+Ferm =Pink |
Staphylococcus epidermidis
Morophology, Media | GPC(clusters)
MSA=+Ferm=yellow
Non Beta Hem |
Tests for Staphylococcus epidermis | Coag -
Susceptible to Novobiocin |
Staphylococcus saprophyticus
Morphology, Media | Non Beta Hem
Coag - |
Test used for
Staphylococcus saprophyticus | Coag -
Resistant to Novobiocin |
Streptococcus pyogenes
Morphology, Media | GPC (chains)
beta Hem on BAP (large zone)
Non beta Hem |
Test for Streptococcus pyogenes
Strep A | Susceptible to A disk (bacitracin)
PYR +
CAMP + |
Streptococcus agalactiae
Strep B
Morphology, Media | GPC (chains)
beta Hem on BAP
small zone |
Streptococcus pneumoniae
Morphology, Media | GP (diplococci)
*alpha hem on BAP* |
Test for Streptococcus pneumoniae | Bile esculin -
susceptible to P disk (optochin)
Bile solubility + |
Enterococcus faecalis
Morphology, Media | GPC (chains)shorts
gamma hem on BAP |
Test for Enterococcus faecalis | Bile esculin +
Salt tolerance +
PYR + |
Positive Bile esculin distinguishes which two organisms? | Enterococcus faecalis from Streptococcus pneumoniae |
Streptococcus bovis
Morphology, media | GPC (chains)
gamma hem on BAP |
Salt tolerance distinguish between which two organisms? | Positive = Enterococcus faecalis
Negative = Streptococcus bovis |
Viridans Streptococcus | GPC (chains) lots
alpha Hem on BAP |
P disk (optochin) test distinguish between which two organisms? | Resistance = Viridans Strep
Susceptible = Streptococcus pneumoniae |
Bile solubility test distinguishes which two organisms? | Positive = Streptocuccus pneumoniae
Negative = Viridans Strep |
A disk (bacitracin) test distinguishes between which two organisms? | Resistance = Streptococcus agalactiae
Susceptible = Streptococcus pyogenes |
Which organism doesn't go on BAP? | Neisseria gonorrhoeae |
Neisseria gonorrhoeae
Morphology, Media | Intercelluar diplococci
ONLY grows on CHOC |
Neisseria gonorrhoeae
Test used? | CTA glucose, Red NET test |
Neisseria lactamica
Gram stain results? | GN diplococci |
Neisseria lactamica grows on what type(s) of agar? | CHOC, MTM, and BAP |
Which organism only grows on CHOC and BAP? | Microcuccus catarrhalis |
Which organism is an asaccharolytic? | Microcuccus catarrhalis |
What organism has negative results for CTA test and NET test? | Microcuccus catarrhalis |
Escherichia coli gram stain result? | GNR |
Which organism is a positve fermenter on MAC and beta hemolytic on BAP? | Escherichia coli |
What is the IMViC for E.coli? | ++-- |
What is the TSI result for E. coli? | A/A H2S- |
What are the distinguishing characteristics for E. coli? | Flat colonies with green metallic colonies on EMB |
How to distinguish E.coli(fermenter) from non fermenting E.coli? | Non fermenting E.coli does not ferment on MAC agar it appears clear and with the TSI the tube will show K/A |
Enterobacter aerogenes IMViC ? | --++ |
Is Enterobacter aerogenes a fermenter? | No |
What is the TSI result for E.aerogenes? | A/A |
Which amino acid(s) does E. aerogenes result(s) is/are positive? | Lysine and Ornithine |
Which amino acid(s) does E. pneumoniae result(s) is/are positive? | Lysine |
Is E. pneumoniae a fermenter? | Yes on MAC |
How does Proteus mirabilis look with gram staining? | GNR |