Term | Definition |
Giesma for H. Pylori | stains for helicobacter pylori
diff quick solution I
diff quick solution II
Acetic water, then check with microscope |
Giesma for H. Pylori | H. pylori.................DARK BLUE
Other bacteria.......BLUE
Nuclei....................DARK BLUE
Cytoplasm...............PINK |
Brown-Hopps Modified Gram Stain | stains gram positive and negative bacteria
crystal violet (gram positive)
gram iodine(mordant)
blot paper, acetone (decolorize neg)
basic fuchsin->gallego->acetone->picric acid acetone |
Brown-Hopps Modified Gram Stain | Gram (+).........BLUE
Gram(-)...........RED
Background.....YELLOW
Nuclei..............LIGHT RED |
Auramine-Rhodamine Fleorescene | stains for Mycobacterium Tuberculosis or other AFB
Auromine-Rhodamnie B solution-> acid alcohol-> Eriochrome Black T |
Auramine-Rhodamine Fleorescene | AFB..................reddish yellow fluorescene
Background......BLACK |
Fite Acid Fast Stain for Leprosy | demonstrates mycobacterium leprae
xylene with peanut oil-> ZN carbol fuchsin-> acid alcohol-> methyl blue |
Fite Acid Fast Stain for Leprosy | M leprae...............RED
Background...........BLUE |
Acid Fast Kinyoun Acid Fast Stain | Kinyoun Carbol-Fuchsin -> Acid Alcohol (to differentiate)-> Methylene Blue (counterstain)
AFB....................BRIGHT RED
Background.........BLUE |
Ziehl-Neelsen for AFB | Ziehl Neelson Carbol Fuchsin (uses ethyl alcohol)
acid alcohol-> methylene blue
AFB....................BRIGHT RED
Background.........BLUE |
Hotchkiss-Mcmanus PAS for Fungus | demonstrates fungus
Fungi.................ROSE
Background.......GREEN |
FUNGUS STAINS | PASF
CHROMIC ACID-SCHIFF STAIN
GRIDLEY
GROCOTT METHANAMINE SILVER NITRATE |
Grocott Methanamine- Silver Nitrate Fungus Stain | chromic acid is the oxidizer for the fungal cell wall
stains for pneumocystis jirovecii
chromic acid->sodium bisulfate-> pre-heated methanamine silver-> gold chloride-> sodium thiosulfate -> light green |
Grocott Methanamine- Silver Nitrate Fungus Stain | Fungi......................BLACK
Mucin......................TAUPE
Background..............GREEN |
SPIROCHETE STAINS | WARTHIN-STARRY
DIETERLE
STEINER & STEINER |
Warthin-Starry for Spirochetes | 2% Silver nitrate (developer)
1% Silver nitrate (impregnation)
Developer (gelatin, hydroquinine, 2% silver nitrate) |
Warthin-Starry for Spirochetes | Spirochetes & bacteria.........BLACK
Background..........................YELLOW/BROWN |
Steiner | demonstrates spirochetes, helicobacter, and legionella
uses gum mastic |
Steiner | Spirochetes, H. pylori, L. Pneumophila,
other nonfilamentous bacteria...............DARK BROWN TO BLACK
Background............................................LIGHT YELLOW |