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Genetics Exam5 Ch19
Probable exam questions for Ch 19 Introduction to Genetics
Question | Answer |
---|---|
Genetic engineering manipulates gene products at the level of: A.the Protein B Amino Acid C. DNA D. RNA | C. DNA Biotechnology that manipulates genetic material altering DNA of an organism to supress or enhance the activities of its own genes and/or genes of different species. Organisms that harbor DNA are transgenic and their DNA is recombinant DNA |
A molecule that consists of a piece of DNA from one organism combined with the DNA from member of another species is called: A. Restricted DNA B. Recombinant DNA C. Bioengineered DNA D. Mutant DNA | B. Recombinant DNA p. 376 |
In 1975, scientists convened in Asilomar California and did what? | Drew up guidelines to regulate recombinant DNA technology p.379 |
Proteins isolated from bacteria that catalyze specific cleavage of DNA are: A. Restrictive enzymes B. Bacteriophage enzymes C. Plasmids | A. RESTRICTION ENZYMES |
Restriction enzymes cut DNA at: A. The sequence CTGGTC only B. A site specific for each enzyme C. Specific short methylated sequences | B. SITE SPECIFIC FOR EACH ENZYME A natural function of enzymes is to protect bacteria by cutting DNA of infected viruses.-Methyl groups shield DNA from restriction enzymes.-Make sticky ends in DNA by cutting at specific sequences-GAATTC cut between G&A |
A small circular DNA molecule used as a vector to transmit foreign DNA is a: A. Plasmid B. Prion C. Liposome | A. Plasmid p.380 |
Bacteriophages can be used as a vector in recombinant DNA experiments because they: | C. Insert their genetic material into bacteria Bacteriophages can be vectors. They transport DNA but don't cause disease.....Virus that infect bacteria |
Biotechnology is possible because the genetic code is universal. True or False | TRUE Creating transgenetic organisms is possible because all life uses the same genetic code. p376 |
The first drug produced using recombinant DNA technology was: A. insulin B. Streptokinase C. TPA D. EPO | A. insulin Drugs are pure, human versions of protein, HGH, insulin, FSH and clotting factors. First great use-Drug invented was insulin in 1982. Humulin made by E. Coli, interferon B-1b. |
A drug produced using recombinant DNA technology that is used illegally by atletes is: A. insulin B. Streptokinase C. TPA D. EPO | D. EPO (erythropoietin) |
True or False? Every gene that is sequenced can be patented? | FALSE Because of the flood of applications a DNA sequence alone doesnt get patent protection p 377 |
A multicellular organism that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is a: A. Transversion B. Transisition C. Transgenic | C. Transgeneic |
Tiny fat bubbles used to deliver genes are: A. Electropores B. phosholipids C. liposomes | C. liposomes p 385 |
Which of these use a tiny needle to inject DNA into cells (microinjection)? A. Electrophoresis B. Microinjection C. Particle bombardment | MICROINJECTION p.386 |
To create a transgenic organism: A. Introduce foreign DNA into a gamete or fertilized ovum B. Inject a gene of interest into somatic cells C. Inject a gene of interest into several somatic cells | A. Introduce foreign DNA into a gamete or fertilized ovum. |
Which of these are used to introduce DNA into animal cells: A. Liposomes B. Electroporation C. Microinjection D. Particle bombardment E. All of these | E. ALL OF THESE p. 385-386 lists these |
Which of these would NOT be used to introduce DNA in animal cells? a. Liposomes B. Electroporation C. Microinjection D. Ti plasmid | D. Ti Plasmid Ti is a plant vector (tumor inducing) Plants, Ti causes tumor like growths. Ti mixed with bt can produce corn which makes its own pesticide. 2/3 all corn in the USA. |
Transgenic organisms carry the transgene in: A. Every Cell B. Gametes only C. Somatic cells only D. The cell that originally gets it | A. EVERY CELL |
BIOREMEDIATION USES BACTERIA OR PLANTS TO DETOXIFY POLLUTANT A. TRUE B. FALSE | A. TRUE p. 386 |
DNA that is not associated with protein is called: A. Recombinant DNA B. Naked DNA C. CDNA D. Digested DNA | B. Naked DNA |
The Ti plasmid is used to genetically engineer: A. Bacteria B. Fungi C. Plants D. Animal | Hint- if you see Ti think Plants. C. PLANTS |
TRANSGENIC PHARMING involves using _______ to produce recombinant proteins in _____ | Cows, Milk p. 385 |
Bt toxin producing plants are resistant to: A. fungal pathogens B. Bacterial patholgens C. Herbicides D. Insect pests | INSECT PESTS Potato/Beetles also have a Ti pesticde or insecticide gene. Ti plasmids and bacillus Thuringiensis bt produce corns with pesticides. |
Nonheritable gene therapy is perfprmed on________ cells. A. Germline B. Somatic C. Constitutional | B. SOMATIC I THINK this is the manipulation of gene expression in cells that will affect the patient but they can't pass it to their children. It can alter DNA of a fertilized egg, It's nonheritable! Corrects only defective/broken cells |
A bone marrow transplant is an example of: A. Germline (heritable) B. All heritable gene therapies C. Somatic gene therapy D. In Vitro fertilization | SOMATIC GENE THERAPY |
Which is an example of GERMLINE THERAPY? | Transgenic plant or animals aka modified plants and animals! Introduces genetic change into sperm/fertilized egg. It's heritable, Alters DNA of the fert. egg, Used to pre-treat embryo and used to treat germ cells. |
Which cell type in the human body would not be a direct target for gene therapy? Can you guess why? A. Liver cells B. Red Blood Cells C. skin cells D Muscle cells | RED BLOOD CELLS- dont have a nucleus so gene therapy probably isn't good. |
Muscle is a good target for gene therapy because: | It is an abundant tissue with a rich blood supply. ( Good site for somatic gene therapy my guess) |