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MICRO2
The control of microbial growth: Sanitation, disinfectants, and sterilization
| Question | Answer |
|---|---|
| BASICS OF MICROBIAL CONTROL | BASICS |
| Disinfection? | Reducing of inhibiting microbe growth on object |
| Sepsis: | there's a bacteria present |
| Asepsis: | No bacteria present |
| Antisepsis/aseptic? | Reducing/inhibiting microbial growth on LIVING tiesue |
| --Cide suffic: | To kill |
| --state suffix: | inhibit |
| HEAT | HEAT |
| What are methods of killing using moist heat? | Boiling and steam under pressure |
| What do you need to get steam under pressure? | Autoclavve |
| What does this method sterilzize? | We sterilize liquids with this method |
| What MUST you have for this to work? | Steam must contact organism |
| How much liquid can we sterilize withthis method? Why? | Small amounts, otherwise you need a long exposure time |
| PASTEURIZATION | PASTEURIZATION |
| What does pasteurization result in? | Kills pathogens, but not all microbes |
| What is a sterilized solutioN/ | When everything is dead |
| When is pasteurization used? | To prevent spoilage, |
| What is the classical method for sterilization? | 63 degrees for 30 mins; not complete sterlization |
| What is the method for commercial use? | 72 degrees for 15 secs |
| What does the ultra high method result in? What undergoes this? | Complete sterilization; coffee creamers |
| FILTRATION | FILTRATION |
| What are the two methods? | Air and water |
| What do we use to air filter stuff? | Vacuums, HEPA filter |
| How do we filter fluids? Disadvantages? | Slow filtration, you must know bacteria size or it can pass through membrane filter |
| HEAVY METALS: What is oligodynamic action/ | Cystine action/ combing with sulfhydryl groups to denature proteins |