Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
MICRO2
Microbial Engineering
| Question | Answer |
|---|---|
| Fig. 9.2 | Restriction Enzymes (2 Figures) |
| Fig. 9.4 | PCR (2 Figures) |
| Fig. 9.16 | Southern Blotting |
| What are the steps of generalized transduction? FIRST STEP ONLY PLEASE | Bacteriophage attaches to cell wall and injects its DNA to bacteria |
| What is the second step of transduction? | Phage DNA and proteins are made from DNA released and bacterial chromosome is broken into pieces by phage enzyme |
| THIRD POSSIBLE STEP? | (Bacterial DNA gets into phage capsid), then the donor cell lyses and relases phages |
| STEP 4 | Phage infects new host cell |
| Step 5 | REcombination can occur, can produce recombinant cell with a genotype different from botth donor and recipient |
| Why would specialized transduction occur? | When phage wants something specific from the bacteria, such as a toxin |
| What is the core difference between the specialized vs. generalized transduction? | The fact that a specific DNA region is transducted. |
| GENETIC ENGINEERING | GENETIC ENGINEERING |
| Thus, what are two key tools in genetic engineering? | Restriction enzymes and cloning plasmids |
| What are restriction enzymes? | DNA cutting enzymes in bacteria that recognizes and cuts only one particular sequence of nucleotide bases in DNA and it cuts them the same way each time |
| What is the process of restriction enzyme in making rDNA? What is needed to form rDNA? | The restriction enzyme cuts the double stranded DNA at a speciific site -->sticky ends-->identical sets join by base pairing --> make rDNA by DNA ligase |
| PCR | PCR |
| What kind of enzyme is used to copy DNA in PCR? What kind of bcteria is needed? | DNA polymerase from thermophillic bacteria that can survive extreme 94 degree heat |
| What is the process of PCR? | 1) Get DNA, 2) Melt it w/ heat stable polymerase 3) Add primers 4) Add heat to melt DNA strand together 5) Repeat |
| What are primers? Why do we need them? | Sequences of DNA specfic to gene that will indicate WHAT we will be copying |
| What is PCR? | Making a lot of DNA from little starting material |
| What do we do when we get the gene that we want? Where do we put them? | Bacterial fingerprinting to see if bacteria are related, Gene therapy |
| Is Gene therapy a successful idea? | Nope... |
| DNA probes? | Short segments of single stranded DNA that are complementary to the desired gene |
| What is southern blotting? | Detection of DNA on filter q/ restriction enzymes without much radioactive probes |
| What has replaced the process of BActerial DNA fingerprinting? | PCR reaction |
| Introns vs. extrons and cDNA | Figure 9.9 |
| How do you make cDNA? | 1. RNA polymerase transcribes DNA w/ introns and extrons-->RNA 2. Remove introns 3. Isolate mRNA (add RT) 4. Make first DNA 5. Make second 6. RT digests mRNA (making cDNA w/ no introns) |
Created by:
talkglitter2486