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ASCP micro study
study guide for micro at sanford brown
| Question | Answer |
|---|---|
| Children who have infections with beta-hemolytic streptococci can develop: | acute glomerulonephritis |
| An unusual number of MRSA (determined by the Bauer-Kirby method) were isolated in the laboratory in the past month. Which of the following is the most likely explanation? | deterioration of the methicillin disks |
| A Gram-stained sputum smear revealed 25-50 squamous epi and 10-25 polymorphonuclear leukocytes, as well as many lancet-shaped, GPC; many GNR and many GPC in pairs, clumps and long chains. the tech's best course of action would be to: | call the nursing station and request a new specimen |
| A GPC isolated from blood culture has the following characteristics: optochin-neg, bacitracin-neg, bile esculin-neg, hippurate-pos, catalase-neg, This organism is most likely: | Streptococcus agalactiae (group B) |
| The function of N-acetyl-L-cysteine required in the NALC-NaOH reagent for acid-fast digestion-decontamination procedures is to | liquify the mucus |
| Middlebrook 7H10 and 7H11 media must be refridgerated in the dark and incubated in the dark as well. If these conditions are not met, the media may prove toxic for mycobacteria because: | formaldehyde may be produced |
| Group B, beta-hemolytic streptococci may be distinguished from other hemolytic streptococci by which of the following procedures? | coagglutination |
| Which of the following media is routinely used to culture Campylobacter jejuni? | Skirrow's medium |
| Which of the following organisms must be incubated in a microaerophilic environment for optimal recovery of the organism? | Campylobacter fetus |
| A cell culture line commonly used for the recovery of chlamydia trachomatis from clinical specimens is: | McCoy's |
| In a disk diffusion susceptibility test, which of the following can result if disks are placed on the inoculated media and left at room temperature for an hour before incubation? | zones of larger diameter would result |
| Faiure to obtain anaerobiosis in anaerobe jars is most often due to the: | inactivation of the palladium-coated alumina catalyst pellets |
| A beta-hemolytic, catalase positive, coccus is coagulase-negative by the slide coagulase test. Which of the following is the most appropriate action in identification of this organism? | do a tube coagulase test to confirm the slide test |
| When combined antimicrobial drugs are clearly less effective than the most active drug alone, the condition is described as: | antagonism |
| The smallest concentration of antimicrobial agent that prevents growth in subculture or results in a 99.9% decrease of the initial inoculum is the definition of: | minimum bactericidal concentration |
| If the effect of combined antimicrobials id greater that the sum of the effets observed with the two drugs independently, the condition is described as: | synergism |
| The smallest amount of test antimicrobial that will inhibit visible growth of a microbe is the definition of: | minimal inhibitory concentration |
| Beta-hemolytic streptococci that are bacitracin-resistant and CAMP-positive are: | group B |
| Beta-hemolytic streptococci that are bacitracin-resistant and CAMP-negative are: | group A |
| Which of the following reagents shold be used as a mucolytic, alkaline reagent for digestion and decontamination of a sputum for mycobacterial culture? | N-acetyl-L-cystine and NaOH |
| It is important to identify individual members of the group D streptococci because: | enterococci often show more antibiotic resistance than other group D streptococci |
| The most critical distinction between Staphylococcus aureus and other Staphylococci is: | coagulase production |
| Optimum growth of Campylobacter jejuni is obtained on suitable meia incubated at 42^C in an atmosphere containing: | 6% O2, 10%-15% CO2, 85%-90% nitrogen |
| Vibro parahaemolyticus can be isolated best from feces on: | thiosulfate citrate bile salts (TCBS) agar |
| Of the following bacteria, the most frequent cause of prosthetic heart valve infections occurring within two to three months after surgery is: | Staphylococcus epidermidis |
| Which of the following would best differentiate Streptococcus agalactiae (Group B) from Streptococcus pyogenes (Group A)? | hydrolysis of sodium hippurate |
| Characteristically, group D enterococci are: | relatively resistant to penicillin |
| The best medium for culture of Mycobacterium tuberculosis is: | Lowenstein-Jansen's |
| An important cause of acute exudative pharyngitis is: | Streptococcus pyogenes |
| CSF from a febrile 25-yr-old man with possible meningitis is rushed to the lab for a stat Gram stain and culture. Performing the Gram stain the tech spills most of the specimen. The smear shows many neutrophils & no microorganisms enough 4 1 plate? | chocolate agar plate |
| When processing thraot swabs for a strep culture, the medium of choice is: | sheep blood agar plates |
| A throat swab is submitted for anaerobic culture. This specimen should be: | rejected |
| Media used to support growth of Legionella pneumophila should contain which of the following additives? | charcoal and yeast extract. |
| An aspirate of a deep wound was plated on a blood agar plates aerobically &anaerobically. At 24hrs there was growth on the anaerobic plate only. the next step is: | begin organism identification |
| SPS is used as an anticoagulant for blood cultures because it: | inactivates neutrophils and components of serum complement |
| Upon review of a sputum Gram stain, the tech notes that the nuclei of all the neutrophils present in the smear are staining dark blue. Why? | The slide was inadequately decolorized with acetone/alcohol |
| A medium that is used for primary isolation and enhances the growth of a particular organism is called: | enrichment |
| Which of the following groups of specimen would be acceptable for anaerobic culture? | pleural fluid, brain abscess |
| Which of the following is the most appropriate specimen source and primary media battery? | endocervical- chocolate,Thayer-Martin |
| A beta-hemolytic strep that has been isolated from an ear culture grows up to the edge of a 0.04-unit bacitracin disk. what test will determine if its an enterococcus? | growth in 6.5% NaCL broth |
| In a guality control procedure on a new batch of Mueller-Hinton plates using a stock culture of Staphylococcus aureus, all the disk zone sizes are too small. The most likely reason for this is that the: | bacterial suspension was not diluted to the proper concentration |
| Three set of blood cultures were obtained. The aerobic bottle of one set had growth of Staphylococcus epidermidis on the 7-day subculture. This inicates that: | the organism is most likely a contaminant |
| The procedure that ensures the most accurate results of oxacillin (methicillin) susceptibility testing against Staphylococci is: | addition of 4% NaCl |
| "Nutritionally deficient" Staphylococci are: | viridans Staphylococci |
| In the optochin (ethylhydrocupreine hydrochloride) susceptibility test, if there is a zone of inhibition of 19 to 30 mm surrounding the disk following overnight incubation at 37^C, the colony most likely consists of: | pneumococci |
| Proper media for culture of a urethral discharge from a man include: | chocolate and modified Thayer-Martin agars |
| Infection of the urinary tract is most frequently associated with: | Escherichia coli |
| Which of the following clean voided urine culture counts indicates the paitient probably has a urinary tract infection? | 10^5 CFU/mL |
| A gamma-hemolytic Streptococcus, which blackens bile esculin but does not grow in 6.5% NaCl broth, is most likely group: | group D nonenterococci |
| The expected colony count in a suprapubic urine from a healthy indivisual is: | 0 CFU/mL |
| Gram-positive cocci in chains are seen on a Gram stain from a blood culture. Further tests should include: | bile esculin, bacitracin, and hippurate |
| An aspirate of a deep wound was plated on blood agar plates aerobically and anaerobically. At 24 hours there was a growth on both plates. This indicates that the organism is: | facultatively anaerobic |
| A urine Gram stain shows gram-positive cocci in clusters. To SPECIATE this organism from culture, the technician should perform a coagulase test and a: | novobiocin inhibition |
| The optimal incubator temperature for isolation of the Campylobacter jejuni/coli group is: | 42^C |
| A tech is asked to clean out the chemical reagent storeroom and discard and reagents not used in the past 5 years. How should the tech proceed? | consult MSDS sheets for proper disposal |
| Media for screening suspects cases of hemorrhagic E coli 0157:H7 must contain: | sorbitol |
| Which selective medium is used for the isolation of gram-positive microorganisms? | Columbia CNA with 5% sheep blood |
Created by:
lesliederby
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