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Protein Structure
Enzymatic cleavage for protein determination
Question | Answer |
---|---|
Trypsin | Lysine (K), Arginine (R) - C terminus |
Chymotrypsin | Tyrosine (Y), Tryptophan (W), Phenylalanine (F), Leucine (L ) - C terminus |
Thermolysin | Isoleucine (I), Valine (V) - N terminus |
Pepsin | Tyrosine (Y), Tryptophan (W), Phenylalanine (F), others - N terminus |
Staph. aureus (V8 Protease) | Glutamate (E), Asparatate (D) - C terminus |
Myobacter protease | Lysine (K) - N terminus |
Papain | Arginine (R), Lysine (K) - fast C terminus; Glutamic acid (Q), Histidine (H), Glycine (G), Tyrosine (Y) - slow C terminus |
Subtilism | Glutamine (Q), Serine (S), Leucine (L), Tyrosine (Y), Phenylalanine (F) - C terminus |
Pronase | Many |
Lys C | Lysine (K) - C terminus |
Arg C | Arginine (R) - C terminus |
Carboxypeptidase A | Non-polar residues |
Carboxypeptidase B | Lysine (K) and Arginine (R) |
Carboxypeptidase C | All residues at different rates |
Carboxypeptidase Y | Hydrophobic (faster), hydrophilic (slower) |
Dipeptidyl aminopeptidase | Dipeptides |
Leucine aminopeptidase | Preferentially hydrophobic |
Aminopeptidase M | Broader specificity |
Met-X | Cyanogen Bromide |
Arg-X | Trypsin (also cleaves lysine) |
Glu(E)-X | S. aureus V8 protease (also cleaves Asp (D)) |
Try(W)-X | BNPS |
Lys(K)-X | Endo Lys C Protease |
Arg(R)-X | Endo Arg C Protease |
Asp(D)-Pro(P) | Weak acid, takes days |
Asn(N)-Gly(G) | Hydroxylamine |
Hydrophobic | Chymotrypsin, Pepsin, Thermolysin, Subtilisin, Papain |