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Micro Lab Part 1
Lab 6 Motility Determination; Cultivation Anaerobes
Question | Answer |
---|---|
What is the role of oxygen for cellular respiration? | Oxygen acts as a terminal electron acceptor |
What type of metabolism occurs in the absence of oxygen? | Anerobic respiration or fermentation |
Name two enzymes that are present in obligate aerobes but lacking in obligate anaerobes. What is the function of each enzyme | Obligate anaerobes lack superoxide dismutase, which converts toxic superoxide to a harmless compound, and catalase, which converts toxic peroxide to oxygen |
Differential between a microaerophile and an aerotolerant organism | Microaerophiles requ low amts of oxygen for metabolism, higher amts are lethal. Aerotolerant org can grow in oxygen but do not use it for metabolism & are indifferent to its presence |
Why is resazurin a useful media additive for the study of anaerobes? | It is an indicator for the presence of oxygen |
Growth only at top of FTM is; | obligate aerobe |
Growth only at bottom of FTM is; | obligate anaerobe |
Even growth throughout FTM tube is: | aerotolerant |
Heavy growth at top, lighter growth at bottom of FTM tube is: | facultative anaerobe |
Growth only in the middle of the FTM tube is; | Microaerophile |