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hematology methodolo
| Term | Definition |
|---|---|
| the Coulter principle | -electrical conductivity or radio frequency -optical scatter -light absorption -fluoresces |
| VSC | volume, conductivity, scatter |
| hydrodynamic focusing | both optical and impedance methods of cell counting employ hydrodynamic focusing |
| electrical impedance principle of counting cells | cells are counted by detecting and measuring changes in electrical resistance when a particle passes through a small aperture -used to count wbc, rbc, and platelets |
| when blood us aspirated, diluted and then divided into 2 samples | -rbc and platelets -wbc and hemoglobin |
| particles between 2 fl and 20 fl are counted as | platelets |
| greater than 36 fl are counted as | rbc |
| cold agglutinins | low red cell count and high MCV can be caused by an increased number of large red cells or red cells agglutinates -present in infectious mononucleosis and mycoplasma pneumonia infection |
| fragmented or very microcytic red cells | -may cause red cell counts to be decreased -may flag the platelet count as the red cells become closer in size -visible at the left side of red cell histogram -right end of platelet histogram |
| platelet clumps and platelets satellitosis | -cause falsely decreased platelet count -can be seen on right side of platelet histogram -confirmed by peripheral smear -scan edge oof smear to check |
| giant platelets | -exceed the size of red cells -cause the right hand of tail histogram to remain elevated -may be seen at left of red cell histogram |
| nucleated red blood cells | interfere with wbc on some instruments by being counted as white cells |
| MPV | mean platelets volume |
| MCV | mean red cell volume |
| MCH | mean cell hemoglobin |
| MCHC | mean cell hemoglobin concentration |
| RDW | red cell distribution width |
| WBC intefering factors | -unusual RBC abnormalities that resit lysis -nucleated RBC -fragmented wbcs -unlisted particles greater then 36 fl -very large plts -specimens containing fibrin, cell fragments, or other debris |
| RBC interfering factors | -very high wbc -high concentration of plts -agglutinated rbc -rbc smaller then 36 fl -specimens containing fibrin, cell fragments, or there debris |
| Hgb interfering factors | -very high wbc -sever lipemia -heparin -certain unusual rbc abnormalities that resit lysing -levels of triglycerides -high bilirubin |
| MCV intefering factors | -very high wbc -high concentration of plts -agglutinated rbc -rbc fragments that fall below 36 fl -rigid rbcs |
| Plt intefering factors | -very small red cell near the upper threshold -cell fragments -clumped plts cellular debris near the lower plt threshold |
| RDW interfering factos | -very high wbc -High concentration of very large or clumped platelets -RBCs below the 36 fL threshold -Two distinct populations of RBCs -RBC agglutinates -Rigid RBCs |
| MPV known factors | platelets count and shape of the histogram -EDTA |
| Hct known factors | parameters used for computation, RBC and MCV |
| MCH known factors | parameters used for computation, Hgb and RBC |
| MCHC known factors | parameters used for computation, Hgb, RBC, and MCV |
Created by:
lovely_lizzie
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