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function of cell stucture NUCLEUS control center
function of cell structure CYTOPLASM all protoplasm other than nucleus
function of cell structure CHROMATIN thread like appearance of the chromosomes when the cell is not dividing
function of cell structure NUCLEOLI oval bodies with in the nucleus direct cell reproduction and protein synthesis
function of cell structure VACUOLE membrane enclosed fluid filled space
function of cell PLASMA MEMBRANE regular passage of materal into and out of cell(some protection and shape)
what size drop of blood is used to make a differtial blood smear blood the size of a match headEDTA Lavender use within 2 hoursfinger puncture do not touch slide with skin
what are the characteristics of a good smear 1)gradual transition from thick to thin end with feathered edge covers 3/4 of slide-2)no waves, holes, clots-3)does not come to abrupt end and does not extend off slide
what are 3 main causes a blood smear to be too thick 1)angle too high-more than 45 degree-2)motion too fast-3)drop too large
what are 3 main causes a blood smear to be too thin 1)drop too small-2)motion too small-3)angle too low
if the blood smears you are making are coming out too thin-how would you correct them 1)more blood-2)increase angle of spreader slide-3)move a little faster
what is the composition of wright's stain alcohol solution-acid dye(eosin-stains reds)-alkaline dye(methylene blue-stains blue)
wright's stain is a differental stain which means causes stains to stain in different colors
what is accomplished in the 2 step staining procedure 1)material fixed to slide with wright's stain-2)polychrome staining with addition to buffer
what controls the acid-base balance the stain 1)low-too red-2)high-too blue-3)ph of the phosate buffer (ph6.4-6.8)
quick stain the stain contains the buffer one step not 2
if the blood smears do not stain well what should you check 1)ph of stain buffer wash water-2)timing
what tests are included in a complete CBC rbc,wbc,platelets
what pathway is followed when counting a differential blood smear serpentine at feathered edge
how many cells are counted 100 consecutive
which microscope objective is used 97x oil immersion
the following cells are not counted when doing a differential blood smear accumulated white cells, disintegrated cells, precipitated stain, smudge cells, basket cells
the following cells are counted when doing a differential blood smear distorted lymphocytes, poorly stained cells, vacuolated cells,
neutrophilic segmented cell function phagocytosis of bacteria and small paricles
neutrophilic segmented cell when increased pyogenic,pyrogenic infection
what are some examples of neutrophilic segmented cells strep throat, bacterial pneumonia, bacterial meningitis, granulocytic leukemia (not bacterial-acute and chronic)
eosinophilic segmented cells function to limit effects of an allergic reaction
eosinophilic segemented cells when increased allergy and parasitic infection
what are some examples of eosinophilic segmented cells asthma, hayfever, parasites(tapeworm, amebic dysentary(causes diarrhea-parasites in the intestine)
basophilic segmented cell function little known 0-1, granules release histomine and heprin, intesify response immediately
basophilic segmented cell when increased granulocytic leukemia, polycythemia vera-role in allergy small
monocyte function phagocytosis of bacteria and large particles
monocyte when increased chronic infection
the following are all non-specific monocyte, basophilic segmented cell, eosinophilic segemented cells, neutrophilic segmented cells
the following are specific lymphocyte
lymphocyte function produce antibodies, transplant rejection
lymphocyte when increased viral infections
examples of lymphocyte mono, chicken pox, things caused by virus
how is neutrophilic segmented cell filament defined so narrow there is no nuclear material between the 2 margins
what color is the cytoplasm in a neutrophilic segemented cell pink with small pink-brown granules
what does the nucleus look like in neutrophilic band cell- C,S, or U shaped-connecting strip wide enough to show nuclear material between margins
what color is the cytoplasm in a neutrophilic band cell same as neutrophilic segmented cell
what do the granules in the cytoplasm look like in eosinophilic segmented cell bright pink-red or red-organs spherical granules fill the cell -uniform size
what do the granules in the cytoplasm look like in basophilic segmented cell large purple-black or blue-black irregular granules-vary in size, shape, and number (buck-shot)
what does the nucleus look like in a lymphocyte dense and round
size of lymphocytes small, med, and large
what color is the cytoplasm of a lymphocyte robbin's egg blue- cyto is clear
what percent of cells are neutrophilic segmented 50-65%
what percent of cells are neutrophilic band 0-7%
what percent of cells are eosinophilic segmented 1-3%
what percent of cells are basophilic segemented 0-1%
what percent of cells are lymphocyte 25-40%
what different shapes are lymphocytes small narrow-large with few reddish granules-look like a spindle-holly shaped
what does the nucleus of a monocyte look like kidney bean shape-fine open lacey
what does the cytoplasm of a monocyte look like slate gray-like ground glass
what other characteristics might help identify as a monocyte firm cytoplasm-very tiny reddish granules(brickdust)-blunt psuedopod(false foot)-vacuoles
what are the 2 kinds of lymphocytes B-cells and T-cells
2 kinds of B-cells plasma and memory
5 kinds of T-cells helper-killer-suppressor-memory-NK
plasma cell makes antibodies
memory cell life span a day
t-4 helper cell help b cells make antibody and summon killer cell
killer cell=cytoxic kiss of death cell-injects infected cell
t-8 supressor cell calls off the attack
memory cell T-cell
NK-natural killer cell immunologic surveillance-quick response
T-cells live months-years 80% are lymphocytes .
80% are T-cells and 20% are B-cells .
Created by: vinnydog