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Phlebotomy Tests

Comments

TestComment
Lactate (lactic acid) (Lact) Analyze in 15 minutes; draw without tourniquet; place in an ice slurry when collecting in an arterial blood gas syringe; however, follow facility protocol
Blood culture Aseptic technique; do not refrigerate
Blood group and type (ABO and Rh) Blood bank ID
Antibody identification (ID)/screen Blood bank ID
Type and screen Blood bank ID
Crossmatch Blood bank ID remains on 72 hours
Cryoglobulin Collect in prewarmed tube and keep at 37°C
Calcium Deliver to the laboratory immediately; refrigerate
Ethanol/alcohol (ETOH) Do not open tube until testing; may require chain of custody; only use nonalcohol solution for skin cleansing
Electrolytes (Lytes) Na, K, Cl, CO2 Do not refrigerate specimen
Carbon monoxide (CO) Do not underfill; refrigerate immediately
Aluminum (Al) Do not use gel
Lithium (Li) Draw 12 hours post dose
Fasting blood sugar (FBS) Ensure patient has fasted for 8 hours before blood collection
C-peptide Ensure patient has fasted; avoid hemolysis
Adrenocorticotropic hormone (ACTH) Freeze plasma
Antidiuretic hormone (ADH) Freeze plasma
Antithrombin III Freeze plasma immediately
Viral load Freeze plasma immediately
Plasminogen Freeze plasma immediately
Acid phosphatase Freeze serum
Partial thromboplastin time (PTT); activated partial thromboplastin time (APTT) Full tube; nonheparinized samples are stable at RT up to 4 hours; heparinized samples must be centrifuged within 1 hour and are stable up to 4 hours
Prothrombin time (PT) Full tube; stable at RT up to 24 hours
Differential (Diff) Make blood smear within 1 hour of collection
Cold agglutinins Must be kept at 37°C; no gel barrier tubes
Carbamazepine (Tegretol) No gel barrier tubes
Antibiotic assays (amikacin, gentamicin, theophylline, tobramycin, vancomycin) No gel barrier tubes
Drug screen No gel barrier tubes
Cyclosporine No gel barrier tubes
Salicylate (aspirin) No gel barrier tubes
Valproic acid (Depakote) No gel barrier tubes
Therapeutic drugs (digoxin, theophylline [theo], phenobarbital, phenytoin [Pheny], carbamazepine [Carb], valproic acid [val ac]) No gel barrier tubes; centrifuge and separate within 1 hour
Vitamin A No gel barrier tubes; ensure patient has fasted
Vitamin B6 No gel barrier tubes; ensure patient has fasted; protect from light
Calcitonin No gel barrier tubes; freeze serum immediately
Aldosterone Patient should be lying down for at least 30 minutes before blood collection
Parathyroid hormone (PTH) Place in an ice slurry
Homocysteine (Hcy) Place in an ice slurry
Angiotensin-converting enzyme (ACE) Place in an ice slurry
Vitamin B12 Protect from light
Carotene, beta Protect from light
Bilirubin, total and direct (Bili) Protect from light
Porphyrins Protect from light
Cancer antigen (CA-125) Refrigerate
Antistreptolysin O (ASO) titer Refrigerate immediately or freeze serum
Ammonia (NH3) Send in an ice slurry
pH Send on ice slurry
Chromium (Cr) Separate and refrigerate immediately
Cortisol Serum only; timed specimen (a.m.)
Brain natriuretic peptide (BNP) Stable for 4 hours
MI panel (CK isoenzyme [CK-MB], myoglobin [Myo], troponin [Tn]) Stable for 4 hours
Gastrin Transport in an ice slurry
Erythrocyte sedimentation rate (ESR) Tube must be filled at least half-full
Fibrinogen Tube must be full
International normalized ratio (INR) Tube must be full
Factor assays Tube must be full
Ionized calcium (iCA2+) Tube must be full
D-Dimer (D-DI) Tube must be full (stable for 4 hours)
Fibrin degradation product (FDP) Tube will only fill to 2 mL and should clot immediately
Created by: cvis
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