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Microbiology Exam I

Microbiology 2401

QuestionAnswer
Bacteria single-celled or noncellular spherical or spiral or rod-shaped organisms lacking chlorophyll that reproduce by fission
Algae primitive chlorophyll-containing mainly aquatic eukaryotic organisms lacking true stems and roots and leave
Fungi the taxonomic kingdom including yeast, molds, smuts, mushrooms, and toadstools; distinct from the green plants
Viruses ultramicroscopic infectious agent that replicates itself only within cells of living hosts; many are pathogenic
Protozoa Any of the diverse group of eukaryotes, of the phylum Protozoa, that are primarily unicellular, existing singly or aggregating into colonies, are usually nonphotosynthetic
Bacteriophage A virus that infects bacteria
Cell theory the theory that cells form the fundamental structural and functional units of all living organisms
Germ theory of disease also called the pathogenic theory of medicine, is a theory that proposes that microorganisms are the cause of many diseases
Koch's postulates The microorganism must be seen in all cases of the disease, and must be isolated and grown in a laboratory; then must cause the same disease when administered to a test animal; and the same type of microorganism must then be isolated from the animal
Prion an infectious protein particle similar to a virus but lacking nucleic acid
Spontaneous generation the theory, now known to be invalid, that plants, animals and microorganisms arose suddenly from non-living materials under certain environmental conditions
Aseptic technique Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cell, tissue and organ cultures
Anionic Dye A dye that dissociates in aqueous solution to give a negatively charged coloured ion
Compound light microscope an instrument to see objects too small for the naked eye
Differential stain more than one substance used to impart color to tissues or cells, to facilitate microscopic study and identification
Simple stain a single dye used to adhere to the cell wall, coloring the cell making it easy to see.
Electron microscope An electron microscope is a type of microscope that uses a particle beam of electrons to illuminate a specimen and create a highly-magnified image
Heat fixation Heat fixation is the procedure used to support a bacterial smear to a glass slide for viewing by a compound microscope
Gram stain method of staining bacteria using a dye called crystal (gentian) violet.The gram-staining characteristics of bacteria are denoted as positive or negative, depending upon whether the bacteria take up and retain (+) the crystal violet stain or not (-)
Immersion oil In light microscopy, oil immersion is a technique used to increase the resolution of a microscope
Index of refraction immersion oil has the same as glass and is used to replace air and prevent refraction at a glasss-air interface.
Negative stain A procedure in which the background is stained whereas the specimen is not
Objective lens The most important part of a compound microscope. It forms the primary image of the object, which is subsequently examined by the Eyepiece. Objectives are usually classified in terms of magnification
Ocular lens The upper lens or eyepiece of a microscope that the viewer looks through
Phase-contrast microscopy technique that manipulates passage of light through transparent specimens to reveal internal features
Reflection light that bounces off of an object
Refraction the change in direction of a propagating wave (light or sound) when passing from one medium to another
Transmission The ratio of the total amount of light passing through the objective lens to the eye
Fluorescence The property of giving off light at a particular wavelength (emission wavelength) when illuminated by light of a different wavelength (excitation wavelength)
Luminescence Emission of light by chemical or electrical means
Scanning electron microscope a microscope that uses a finely focused electron beam scanned across a sample to produce high resolution images
Transmission electron microscope Transmission electron microscopy (TEM) is a microscopy technique whereby a beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through
Smear a thin tissue or blood sample spread on a glass slide and stained for cytologic examination and diagnosis under a microscope
Wavelength The length of a single cycle of a wave, as measured by the distance between one peak or trough of a wave and the next; it is often designated in physics as λ
Wet mount A type of laboratory test in which a sample of a body fluid is preserved on a slide and examined under a microscope for the presence of microorganisms.
Ziehl-Neelsen acid-fast stain It is a special bacteriological stain used to identify acid-fast organisms
Fluorescence microscopy light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes
Atom the smallest component of an element having the chemical properties of the element
Atomic weight the total number of an atoms protons and neutrons
Protons The proton is a subatomic particle with an electric charge of +1 elementary charge. It is found in the nucleus of each atom.
Neutron an elementary subatomic particle with 0 charge and mass found in the nucleus of each atom.
Electron The subatomic particle having a negative charge and orbiting the nucleus
Mole the molecular weight of a substance expressed in grams; the basic unit of amount of substance adopted under the Systeme International d'Unites
Element any of the more than 100 known substances (of which 92 occur naturally) that cannot be separated into simpler substances and that singly or in combination constitute all matter
Molecule a sufficiently stable, electrically neutral group of at least two atoms in a definite arrangement held together by very strong (covalent) chemical bonds
Compound a substance formed by chemical union of two or more elements or ingredients in definite proportion by weight
Ionic bond a chemical bond between ions resulting from the transfer of electrons from one of the bonding atoms to the other.
Covalent bonds a chemical bond that involves sharing a pair of electrons between atoms in a molecule
Hydrogen bonds a chemical bond consisting of a hydrogen atom between two electronegative atoms (e.g., oxygen or nitrogen) with one side be a covalent bond and the other being an ionic bond
Mixture a substance consisting of two or more substances mixed together (not in fixed proportions and not with chemical bonding)
Solution a homogeneous mixture of two or more substance
Solvent a liquid substance capable of dissolving other substances
Solute the dissolved matter in a solution
Acid is traditionally considered any chemical compound that, when dissolved in water, gives a solution with a hydrogen ion activity greater than in pure water, i.e. a pH less than 7.0. Donates a hydrogen ion (H+)
Base an aqueous substance that can accept hydrogen ions. A soluble base is also often referred to as an alkali if hydroxide ions (OH−) are involved.
pH the logarithm of the reciprocal of hydrogen-ion concentration in gram atoms per L; provides a measure on a scale from 0 to 14 of the acidity or alkalinity of a solution (where 7 is neutral and greater than 7 is more basic and less than 7 is more acidic
Carbohydrates essential structural component of living cells and source of energy for animals; includes simple sugars with small molecules as well as macromolecular substances; are classified according to the number of monosaccharide groups they contain
Lipids an oily organic compound insoluble in water but soluble in organic solvents; essential structural component of living cell
Protien any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissue
Primary protein structure The sequence of the different amino acids
Secondary protein structure the alpha helix and the beta-sheet
Tertiary protein structure elements of secondary structure are usually folded into a compact shape using a variety of loops and turn
Quaternary protein structure the interaction between several chains of peptide bonds.
Nucleotides Nucleotides are molecules that, when joined together, make up the structural units of RNA and DNA
Nucleic Acids A nucleic acid is a macromolecule composed of chains of monomeric nucleotides. In biochemistry these molecules carry genetic information or form structures within cells
Exergonic a process accompanied by the release of energy; "diffusion is an exergonic process"
Endergonic a process accompanied by or requiring the absorption of energy; the products of the process are of greater free energy than the reactants
Phospholipid any of various compounds composed of fatty acids and phosphoric acid and a nitrogenous base; an important constituent of membranes
DNA a long linear polymer found in the nucleus of a cell and formed from nucleotides and shaped like a double helix; associated with the transmission of genetic information
RNA a long linear polymer of nucleotides found in the nucleus but mainly in the cytoplasm of a cell where it is associated with microsomes; it transmits genetic information from DNA to the cytoplasm and controls certain chemical processes in the cell
Triaclyglycerol also know as triglycerides, TAG and TG. The triple esters on a glycerol backbone, which may contain one, two or three different fatty acids, are the most common form of natural lipid
Catabolism breakdown in living organisms of more complex substances into simpler ones together with release of energy
Anabolism the synthesis in living organisms of more complex substances (e.g., living tissue) from simpler ones together with the storage of energy
Prokaryotic cell A cell whose genetic material is not enclosed in a nuclear envelope
Eukaryotic cell A cell containing a membrane-bounded nucleus and membrane-bounded organelles
Cell membrane a thin membrane (a double layer of lipids) enclosing the cytoplasm of a cell
Cell wall a rigid layer of polysaccharides enclosing the membrane of plant and prokaryotic cell
Cytoplasm the protoplasm of a cell excluding the nucleus; is full of proteins that control cell metabolism
Flagella a tail-like structure that projects from the cell body of certain prokaryotic and eukaryotic cells
Peptidoglycan aka murein, is a polymer consisting of sugars and amino acids that forms a mesh-like layer outside the plasma membrane of bacteria, forming the cell wall
Lipopolysaccharide aka lipoglycans, are large molecules consisting of a lipid and a polysaccharide joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria
Periplasmic space a space between the inner cytoplasmic membrane and external outer membrane of Gram-negative bacteria or the equivalent space outside the inner membrane of Gram-positive bacteria.
Gram negative bacteria that do not retain crystal violet dye in the Gram staining protocol
Gram positive bacteria that have a single cell wall that does not contain lipopolysaccharide; these bacteria retain a violet stain when rinsed
Fluid mosaic model biological membranes can be considered as a two-dimensional liquid where all lipid and protein molecules diffuse more or less freely
Hydrophobic lacking affinity for water; tending to repel and not absorb water; tending not to dissolve in or mix with or be wetted by water
Hydrophillic having a strong affinity for water; tending to dissolve in, mix with, or be wetted by water.
Nucleoid resembling a nucleus. the region in a procaryotic cell where the dna is located
Endospore A resting structure formed inside some bacteria that is resistant to unfavorable environmental conditions
Cortex the tissue forming the outer layer of an organ or structure in plant or animal
Vegetative cells Cells involved in growth, nutrition, or asexual reproduction, but not sexual reproduction
Atrichous flagella bacteria without flagella
Monotrichous having a single polar flagella at one end
Amphitrichous having two flagella, one at each end
Lophotrichous having two or more flagella at each end
Peritrichous having flagella all over the surface
Chemotaxis movement by a cell or organism in reaction to a chemical stimulus
Phototaxis movement by a cell or organism in reaction to a light stimulus
Conjugation pili a hairlike appendage found on the surface of many bacteria used to attach two cells and furnish a pathway for transferring genetic material
Attachment pili a hairlike appendage found on the surface of many bacteria used to help bacteria adhere to surfaces
Glycocalyx a general term referring to extracellular polymeric material (glycoprotein) produced by some bacteria, epithelia and other cells
Slime layer an easily removed, diffuse, unorganised layer of extracellular material that surrounds bacteria cells
Capsule very large structure of some prokaryotic cells, such as bacterial cells. It is a layer that lies outside the cell wall of bacteria. It is a well organized layer, not easily washed off, and it can be the cause of various diseases
Plasma membrane membrane that separates the contents of a cell from its outside environment; it consists of a double layer of phospholipids; cell membrane
Mitochondria an organelle containing enzymes responsible for producing energy
Chloroplast An organelle found in the cells of green plants, and in photosynthetic algae, where photosynthesis takes place
Cilia a hair-like structure that moves in a waving motion. Cilia are used for motility in some one-celled organisms
Endosymbiotic theory organelles originated as separate prokaryotic organisms that were taken inside the cell as endosymbionts. Mitochondria developed from proteobacteria (in particular, Rickettsiales or close relatives) and chloroplasts from cyanobacteria.
Simple diffusion a net transport of molecules from a region of higher concentration to one of lower concentration
Selectively permeable a barrier that allows some chemicals to pass but not others
Facilitated diffusion a substance not soluble by itself in lipids is transported across a selectively permeable membrane by combining with a transporter (carrier) molecule
Osmosis diffusion of molecules through a semipermeable membrane from a place of higher concentration to a place of lower concentration
Active transport transport of a substance (as a protein or drug) across a cell membrane against the concentration gradient; requires an expenditure of energy
Hypertonic a solution that has a higher solute concentration than the one to which it is compared (usually some kind of cell)
Hypotonic a solution that has a lower solute concentration than the one to which it is compared (usually some kind of cell
Passive transport any form of movement across a membrane that does not require the use of ATP. This includes simple diffusion, osmosis, the use of various channels, and facilitated diffusion
Endocytosis a process whereby cells absorb particles by enveloping them with the help of vesicles formed from the cell wall
Exocytosis the removal of particles by enveloping them in a vesicle and releasing them to the outside wall
Metabolism the sum of all the physical and chemical processes by which a particular substance is handled
Oxidation the addition of oxygen to a compound with a loss of electrons; always occurs accompanied by reduction
Reduction any process in which electrons are added to an atom or ion (as by removing oxygen or adding hydrogen); always occurs accompanied by oxidation of the reducing agen
Autotroph an organism that makes its own food from light energy or chemical energy without eating
Heterotroph An organism which requires an external supply of energy in the form of food as it cannot synthesize its own
Chemoautotroph An organism that obtains its energy from the oxidation of chemical compounds and uses only organic compounds as a source of carbon
Photoautotroph an organism, such as all green plants, that can synthesize its own food from inorganic material using light as a source of energy
Chemoheterotroph An organism that must ingest organic building blocks that they are incapable of creating on their own
Photoheterotroph organisms that use light for energy, but cannot use carbon dioxide as their sole carbon source
Activation energy minimum amount of energy, that must be accommodated to a system in order to start a process, mostly for chemical reactions
Enzyme A protein catalyst that facilitates specific chemical or metabolic reactions necessary for cell growth and reproduction
Cofactor non-protein component(s) needed by an enzyme to be functional; some cofactors are metal ions, others are coenzymes
Coenzyme a small molecule (not a protein but sometimes a vitamin) essential for the activity of some enzymes
Holoenzyme A complete enzyme consisting of the apoenzyme plus a cofactor
Substrate a molecule upon which an enzyme acts
Active site the part of an enzyme or antibody where the chemical reaction occurs
Allosteric site a site other than the protein's active site
Allosteric inhibitor act as 'modulators' in enzyme execution as they can attach themselves to a molecule that will alter the binding Site for the enzyme, rendering it unusable and therefore rendering the enzyme inactive.
Noncompetitive inhibition a type of enzyme inhibition that reduces the maximum rate of a chemical reaction (Vmax) without changing the apparent binding affinity of the catalyst for the substrate
Competitive inhibition a form of enzyme inhibition where binding of the inhibitor to the enzyme prevents binding of the substrate and vice versa
Glycolysis a metabolic process that breaks down carbohydrates and sugars through a series of reactions to either pyruvic acid or lactic acid and releases energy for the body in the form of ATP
Phosphorylation the process of transferring a phosphate group from a donor to an acceptor; often catalysed by enzymes
Fermentation a process in which an agent causes an organic substance to break down into simpler substances; especially, the anaerobic breakdown of sugar into alcohol
Homolactic acid fermentation all two molecules of pyruvate are converted to lactate
Alcoholic fermentation a biological process in which sugars such as glucose, fructose, and sucrose are converted into cellular energy and thereby produce ethanol and carbon dioxide as metabolic waste product
Anaerobe an organism (especially a bacterium) that does not require air or free oxygen to live
Aerobe an organism (especially a bacterium) that requires air or free oxygen for life
Facultative anaerobe an organism, usually a bacterium, that makes ATP by aerobic respiration if oxygen is present but is also capable of switching to fermentation.
Kreb's cycle acetyl CoA is combined with oxaloacetate to form citric acid; the resulting citric acid is converted into a number of other chemicals, eventually reforming oxaloacetate; NADH, some ATP, and FADH2 are produced and carbon dioxide is released
Tricarboxylic acid cycle the citric acid or Kreb's cycle
Electron transport chain A group of electron carriers in mitochondria that transport electrons to and from each other in a sequence, in order to generate ATP
Chemiosmosis the diffusion of ions across a selectively-permeable membrane. More specifically, it relates to the generation of ATP by the movement of hydrogen ions across a membrane during cellular respiration.
Membrane transport the moving of biochemicals and other atomic or molecular substances across biological membranes
Binary fission The method by which bacteria reproduce. The circular DNA molecule is replicated; then the cell splits into two identical cells, each containing an exact copy of the original cell's DNA
Budding reproduction of some unicellular organisms (such as yeasts) by growth and specialization followed by the separation by constriction of a part of the parent
Lag phase The initial growth phase, during which cell number remains relatively constant, prior to the onset of rapid cell division
Log phase second growth period characterized by cell doubling
Stationary phase third growth phase in which numbers level out due to equal growth and death rate
Death phase fourth phase charcterized by declining numbers of bacteria as nutrients are depleted and cells die
Serial dilution the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion
Pour plate method can be used to determine the number of microbes/mL or microbes/gram in a specimen
Colony forming units a measure of viable bacterial or fungal numbers
Direct microscopic count The number of bacteria in a small known volume is directly counted microscopically and the number of bacteria in the larger original sample is determined by extrapolation
Spectrophotometer an instrument used to measure turbidity
Turbidity the cloudiness or haziness of a fluid caused by individual particles (suspended solids) that are generally invisible to the naked eye
Filtration the process whereby fluids pass through a filter or a filtering medium
Physical growth factors pH, temperature, O2 concentration, moisture, hydrostatic pressure, osmotic pressure, radiation
Nutritional growth factors carbon, nitrogen, phosphorus, trace elements, and vitamins
Acidophile an organism that thrives in a relatively acid environment
Neutrophile an organism that thrives in neutral pH environments
Alkaliphile an organism that thrives in a relatively alkaline environment
Psychrophile extremophilic organisms that are capable of growth and reproduction in cold temperatures
Thermophile extremophilic organisms that are capable of growth and reproduction in hot temperatures
Mesophiles an organism that grows best in moderate temperature, neither too hot nor too cold, typically between 15 and 40 °C (77 and 104 °)
Barophile extremophilic organisms that are capable of growth and reproduction under high barometric pressure
Halophile extremophile organisms that thrive in environments with very high concentrations of salt
Plasmolysis the process in plant cells where the plasma membrane pulls away from the cell wall due to the loss of water through osmosis
Sporulation asexual reproduction by the production and release of spores
Pure culture A culture containing a single kind of microorganism
Defined medium A medium whose exact chemical composition is quantitatively known
Complex medium A medium whose exact chemical composition is not quantitatively known
Selective medium a culture medium containing substances that specifically inhibit or promote the growth of some species of microorganisms
Differential medium A medium which is used to differentiate different types of microorganisms based on their different eg colors or shapes of colonie
Enrichment medium a medium of a given composition which permits preferential emergence of certain organisms that initially may have made up a relatively minute proportion of a mixed inoculum.
Stock Culture A culture of a microorganism maintained solely to keep it viable for subculture into fresh medium
Taxonomy the science of the classification of organisms
Binomial nomenclature the system for naming organisms developed by Carl Linnaeus, in which every organism has a generic name and a specific epithet.
Genus A taxonomic rank above the level of species and below family
Species A group of plants or animals having similar appearance; A rank in the classification of organisms, below genus and above subspecies
Domain the highest taxonomic rank of organisms, higher than a kingdom
Kingdom the highest taxonomic group into which organisms are grouped; one of five biological categories
Monera include all the prokaryotic organisms or bacteria. This group of living organisms do not have any internal membranous organelles
Protista eukaryotic one-celled living organisms distinct from multicellular plants and animals: protozoa, slime molds, and eukaryotic algae
Fungi the taxonomic kingdom including yeast, molds, smuts, mushrooms, and toadstools; distinct from the green plants
Plantae the taxonomic kingdom comprising all living or extinct plants
Animalia taxonomic kingdom comprising all living or extinct animals
Phylogeny the sequence of events involved in the evolutionary development of a species or taxonomic group of organisms
Dichotomous key a guide to identification of plants or animals consisting of a series of pairs of questions or descriptions
Tree of life The concept of a many-branched tree illustrating the idea that all life on earth is related
Shrub of life The concept of a many-branched structure illustrating the idea that all life on earth is related
Archaea A kingdom of unicellular microorganisms, many members of which can survive extreme environmental conditions, such as temperatures >100 °C, extremely alkaline or acid environs, and highly osmotic conditions
Phage typing A method of identifying bacteria using specific strains of Bacteriophages
Numerical taxonomy an attempt to classify organisms based on overall similarity, usually in morphology or other observable traits, regardless of their phylogeny or evolutionary relation
Anneal for DNA or RNA to pair by hydrogen bonds to a complementary sequence, forming a double-stranded polynucleotide
DNA hybridization the process of combining complementary, single-stranded nucleic acids into a single molecule. Nucleotides will bind to their complement under normal conditions, so two perfectly complementary strands will bind to each other readily
DNA sequencing sequencing methods for determining the order of the nucleotide bases—adenine, guanine, cytosine, and thymine—in a molecule of DNA
Monoclonal antibody any of a class of antibodies produced in the laboratory by a single clone of cells or a cell line and consisting of identical antibody molecules
Virus ultramicroscopic infectious agent that replicates itself only within cells of living hosts
Obligate intracellular parasite microorganisms that are capable of growing and reproducing inside cells
Capsid the outer covering of protein surrounding the nucleic acid of a virus
Envelope the covering of some capsids used to help viruses enter host cells
Virion a complete viral particle; nucleic acid and capsid (and a lipid envelope in some viruses)
Capsomeres make up the capsid, an outer covering of protein that protects the genetic material of a virus.
Nucleocapsid The RNA/DNA (genetic material/genome) of the virus and the protein layer (capsid) surrounding it
Glycoproteins A protein with covalently bonded carbohydrates
Naked virus a virus that does not posses an envelope. In these viruses the capsid forms the outer layer
Positive sense RNA signifies that a particular viral RNA sequence may be directly translated into the desired viral proteins
Negative sense RNA is complementary to the viral mRNA and thus must be converted to positive-sense RNA by an RNA polymerase prior to translation
RNA virus a virus that has RNA (ribonucleic acid) as its genetic material
Picornavirus non-enveloped, positive-stranded RNA viruses with an icosahedral capsid
Enterovirus any of a group of picornaviruses that infect the gastrointestinal tract and can spread to other areas (especially the nervous system)
Hepatovirus a genus of viruses belonging to the family Picornaviridae. It encompasses the Hepatitis A with one unclassified virus
Rhinovirus any of a group of picornaviruses that are responsible for many upper respiratory infections
Togavirus linear, single-stranded, positive sense RNA, enveloped and forms spherical particles, the capsid within is icosahedral
Flavivirus A family of viruses transmitted by mosquitos and ticks that cause some important diseases, including dengue , yellow fever , tick-borne encephalitis virus, and West Nile fever
Retrovirus any of a group of viruses that contain two single-strand linear RNA molecules per virion and reverse transcriptase (RNA to DNA); the virus transcribes its RNA into a cDNA provirus that is then incorporated into the host cell, Known for HIV
Reverse transcriptase the enzyme produced by HIV and other retroviruses that enables them to direct a cell to synthesize DNA from their viral RNA
Paramyxovirus a group of viruses including those causing mumps and measles
Rhabdovirus any of a group of arboviruses including those causing rabies
Provirus A provirus is a virus genome that has integrated itself into the DNA of a host cell. One kind of virus that can become a provirus is a retrovirus
Orthomyxovirus a group of viruses including those causing influenza, RNA
Filovirus RNA, Thread-like virus including Ebola and Marburg viruses
Bunyavirus RNA,a family of negative-stranded RNA viruses. Though generally found in arthropods or rodents, certain viruses in this family occasionally infect human
Arenavirus Any of several RNA viruses, of the genus RNA, most of which cause severe diseases transmitted by rodents
Reovirus RNA,a family of viruses that can affect the gastrointestinal system (such as Rotavirus)
DNA virus A virus in which the genetic material is DNA rather than RNA. The DNA may be either double- or single-stranded
Herpesvirus DNA, a group of viruses that includes herpes simplex virus types 1 and 2, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpesvirus types 6 and 7, and Kaposi's sarcoma-associated herpesvirus (HHV-8)
Poxvirus DNA, One of a group of viruses that produce spreading vesicular lesions, including smallpox, vaccinia, and molluscum contagiosum
Papovirus the papilloma viruses and the polyomaviruses. Human papilloma viruses (HPV) are the smallest double-stranded DNA viruses
Hepadnavirus a group of animal DNA viruses including the virus causing hepatitis B in humans
Parvovirus any of a group of viruses containing DNA in an icosahedral protein shell and causing disease in dogs and cattle; not known to be associated with any human disease
Emerging virus are a great cause for concern internationally, and the subject of a great deal of concentrated research
Viral replication Adsorbtion, penetration, synthesis, maturation, release
Phage therapy therapeutic use of bacteriophages to treat pathogenic bacterial infections
Eclipse period A phase in the proliferation of viral particles during which the virus cannot be detected in the host
Latent period defines the period from adsorption, penetration and uncoating of the virus until the first progeny virus are released
Plaque assay A widely used approach for determining the quantity of infectious virus
Lysogeny Process by which a viral genome is integrated into that of its host bacterium
Temperate phage the ability of some bacteriophages (notable coliphage λ) to display a lysogenic life cycle
Cell culture the process by which cells are grown under controlled conditions
Cell strain derived either from a primary culture or a cell line by the selection or cloning of cells having specific properties or markers
Cytopathic effect Viruses can infect target cells and cause cell death
Latent viral infection the ability of a pathogenic virus to lie dormant within a cell
Teratogen any agent that interferes with normal embryonic development: alcohol or thalidomide or X-rays or rubella are examples
Carolus Linnaeus the Father of Taxonomy
Robert Whittaker added a fifth kingdom to Copeland's four kingdoms, the Kindom Fungi (single and multi-celluar osmotrophic eukaryotes)
David Bergey Manual of Determinative Bacteriology
Robert Hooke This English naturalist (1635 - 1703) coined the term "cell" after viewing slices of cork through a microscope
Anton van Leeuwenhoek considered the father of microscopy because of the advances he made in microscope design and use
Robert Koch presented his discovery of Mycobacterium tuberculosis, the bacterium that causes tuberculosis, anthrax. Known for Koch's postulates
Ignaz Semmelweis Known for antiseptic and aseptic technique but was considered a lunatic and placed in an asylum.
Joseph Lister Made aseptic technique modern practice
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