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Tissue Processing

ICLS

Infiltration Temperature 52C - 60C
Histopathologic techniques constitute the backbone of diagnostic pathology, enabling histologic diagnosis of diseased tissue possible for proper management. Tissue Processing
Tissue processing order Fixation->(Decalcification)->Dehydration->Clearing->Infiltration->Embedding->Trimming->Section cutting ->Staining ->Mounting ->Labelling ->Filing ->Storage and Storage Control
one of the most common and most reliable (a mechanical processor with an electric motor that drives gears and cams). TECHNICON MONO TISSUE PROCESSOR or TECHNICON
have the advantage of being examined in the living state Fresh Tissue
protoplasmic activities such as motion, mitosis, phagocytosis and pinocytosis to be observed. Fresh Tissue
are frequently used because the chemicals in the tissue process arrests decay Fixed Preserved Tissue
tissue structures can be studied for prolong period compared to fresh ones. Fixed Preserved Tissue
use has been limited because of the fact that tissues examined in the fresh state are not permanent, and therefore, are liable to develop changes. Fresh Tissue
Enumerate the Methods of Fresh Tissue Examination (4) 1. Teasing or Dissociation 2. Squash Preparation (Crushing) 3. Smear Preparation 4. Frozen Section
It is a process whereby a selected tissue specimen is immersed in a watch glass containing isotonic salt solution carefully dissected or separated, and examined under the microscope. TEASING OR DISSOCIATION
It is a process whereby small pieces of tissues, not more than 1mm in diameter, are placed in a microscopic slide and forcibly compressed with another slide or with a cover glass. SQUASH PREPARATION
It is the process of examining sections or sediments, whereby cellular materials are spread lightly over a slide by means of a wire loop or applicator stick or by making an apposition smear with another slide. Smear Preparation
Enumerate the 4 types of smear preparation 1. Streaking 2. Spreading 3. Pull Apart 4. TOUCH PREPARATION (IMPRESSION SMEAR)
With an applicator stick or a platinum loop, the material is rapidly and gently applied in a direct or zigzag line throughout the slide, attempting to obtain a relatively uniform distribution of secretion. Streaking
A selected portion of the material is transferred to a clean slide and gently spread into a moderately thick film by teasing the mucous strands apart with an applicator stick. Spreading
This is done by placing a drop of secretion or sediment upon one slide and facing it to another clean slide. Pull-Apart
It is a special method of smear preparation whereby the surface of a freshly cut piece of tissue is brought into contact and pressed on to the surface of a clean glass slide, allowing the cells to be transferred directly to the slide. TOUCH PREPARATION (IMPRESSION SMEAR)
This method is normally utilized when a rapid diagnosis of the tissue in question is required, and especially recommended when lipids and nervous tissue elements are to be demonstrated. Frozen Section
Thickness of Tissue in Frozen Section: _________________ 10-15 microns
this process requires close cooperation and communication between the clinician, the nursing staff and clerical in the office; and technical staff of the laboratory. Procurement
The procurement of specimens for histologic evaluation consist of the following elements: (5) 1. Full Patient and Specimen Information 2. Request Form 3. Fixative 4. Specimen Submission Type 5. Transportation
All biopsy specimens should be placed in plastic containers dipped in ______________________________________________ prior to being transported to the Histopathologic section. This should be replaced or added with _________ upon arrival to the section. 1. 10% Formalin (10% Neutral Buffered Formalin) 2. 10% Formalin
T or F Squeeze the specimen into an inadequate container False Do not squeeze a specimen into an inadequate container
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