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Connective & Muscle
Connective & Muscle Tissue Stains
| Question | Answer |
|---|---|
| Four Basic tissue groups are? | Epithelium Tissue, Connective Tissue, Nervous Tissue, Muscle Tissue. |
| This tissue group functions to provide structural and metabolic support for the other tissues and organ in the body? | Connective Tissue |
| The three components that make up connective tissue are? | 1.Fibers 2.Cells 3.Amorphous ground substance. |
| The four different connective tissue types are? | 1.connective tissue proper. 2.cartilage. 3.bone. 4.blood |
| Of the four different connective tissue types, what are histologists most interested in? | Connective Tissue Proper |
| The three FIBERS of connective tissue proper are ? | 1.Collagen. 2.Elastic. 3.Reticular |
| Name 7 different cells found in connective tisuue proper? | Fibroblasts, mesenchymal, adipose, mast cells, macrophages, plasma cells, blood cells. |
| This is one of the four tissue groups and is classified by the differences in structure and in function? | Muscle Tissue |
| The 3 types of muscle tissue are? | 1.Smooth. 2.Skeletal(Striated). 3.Cardiac |
| This type of muscle is nonstriated and involuntary? | Smooth muscle |
| This type of muscle is striated and voluntary? | Skeletal or Striated Muscle |
| This type of muscle is striated and involuntary? | Cardiac |
| This muscle type is composed of branching fibers connected by junctions called intercalated discs? | Cardia Muscle |
| This muscle type has large cylindric fibers, multinucleated which are located on the periphery of the fiber? | Skeletal or Striated Muscle |
| This muscle type has cells that are elongated, tapered at the ends and possess a single centrally located nucleus. | Smooth Muscle |
| Referred to as basal lamina, this is found beneath epithelium and separates the epitheliunm from the underlying connective tissue? | Basement Membrane |
| Due to the glycoprotein present, this can also be stained with carbohydrate stains? | Basement Membrane |
| This functions to provide physical support for the epithelium and provides for cell attachment and for ultrafiltration? | Basement Membrane |
| This C.T.stain differentiates between collagen and smooth muscle in TUMORS and identifies increases in collagenous tissue in DISEASES such as cirrhosis of the liver? | Masson Trichrome (For Collagen and Muscle) |
| This C.T.stain differentiates between collagen and smooth muscle and identifies increases in collagenous tissue? | Gomori One-Step Trichrome (For Collagen and Muscle) |
| Theses TWO C.T.stains demonstrates pathologic changes in elastic tissue? | Verhoeff Elasic Stain and Aldehyde Fuchsin Elastic stain |
| When tissue is overstained and then decolorized or differentiated until the desired component contrasts sharply with the background, this is called? | Regressive Staining |
| A prominent C.T. Regressive stain is? | Verhoeff Elastic Stain |
| This stain demonstrates muscle cross-striations and Fibrin? | Mallory PTAH Technique for Cross Striations and Fibrin |
| Mallory PTAH is used as a neuron stain and a Muscle Stain. What two things does Mallory PTAH stain for? | A Neuron stain for GLIAL FIBERS and a Muscle stain for CROSS-STRIATIONS AND FIBRIN. |
| This stain demonstrates RETICULAR FIBERS in tissue sections and can be important in the differentail diagnosis of certain tumor types? | Gomori Stain for Reticular Fibers |
| This stain demonstrates the glomerular BASEMENT MEMBRANE and is a POLYCHROMATIC Stain? | (PAMS) Periodic Acid-Methenamine Silver Microwave |
| This is a stain for neutral lipds in frozen tissue? | Oil Red O |
| This is a stain for neutral lipds in frozen tissue that is more sensitive than Oil Red O? | Sudan Black B |
| This stain demonstrates MAST CELLS? | Toluidine Blue |
| This is a Nuclear Stain to differentiate between DNA and RNA and is also a connective Tissue Stain. What is the stain and what does it stain for as a C.T. stain? | Methyl Green Pyronin Y and is used to identify plasma cells and immunoblasts. |
| Both frozen Neutral lipid stains, Oil Red O and Susan Black B cut at what micron? | 10 |
| Methyl Green Pryronin Y for both Nuclear and Connective Tisse cuts at what micron? | 4 |
| PAMS (Periodic Acid-Methenamine Silver for the demonstration of Basement Membrane cuts at what micron? | 2 |
| Mallory PTAH cuts at what micron for muscle cross-sriatations and at what micron for glial fibers? | 4-6 microns for Muscle and 6-8 microns for Nervous Tissue. |
| With what stain is Bouin Solution used as a mordant? | Gomori-One Step Trichrome (for collagen and muscle) |
| 10% NBF is preferred for this stain but B-5, Helly or Zenker are satisfactory? | Methyl Green-Pyronin Y |
| NO ALCOHOLIC FIXATIVES can be used with these two lipid stains? | Oil Red O and Sudan Black B |
| Staining results for Oil Red O and Sudan Black B are what colors for fat and what colors for Nuclei? | Fat is intense red and nuclei is blue for Oil Red O and fat is blue/black and nuclei is red for Sudan Black B |
| What color is the cytoplasm, keratin and muscle fibers stained with a Masson Trichrome Stain? What color is collagen and mucus? | Red, Blue |
| What color is the cytoplasm, keratin and muscle fibers stained with a Gomori One Step Trichrome? What color is collagen and mucus? | Red, green or blue |
| What is the control used for the two Trichrome Stains (for collagen and muscle), Masson and Gomori One-step? | Nearly every tissue hasd an internal control but if a control is desired, uterus, small intestine, fallopian tube or appendix. |
| What is the control for the two elastic stains, Verhoeff and Aldehyde Fuchsin? | Oarta embedded on edge or a cross section of large artery. |
| What is the control used for A Mallory PTAH stain that demonstrates muscle cross-striations and fibrin? | Skeletal or cardiac muscle for cross-striations. A section containing fibrin for fibrin demonstration. |
| What is the control for Gomori Stain for reticular fibers? | Liver |
| What is the control for the polychromatic stain Periodic Acid Methamine Silver (PAMS)? | Kidney (2 microns) |
| What is the control used for the fat stains, Oil Red O and Susan Black B? | Most tissue contains fat so normally a control is not used. |
| What is the control used for Methyl Green Pyronin Y for Plasma cells? | A section containing many plasma cells (same as for Methyl green Pyron Y for DNA/RNA) |
| What is the control used for Toluidine Blue for Mast Cells? | A section containing mast cells |
| The principle for this stain is where three dyes which may or may not include the nuclear stain are used? | Masson Trichrome |
| In this stain, a plasma stain (chromotrope 2R) and a connective tissue fiber stain (fast-green FCF, light green, or aniline blue) are combined in a solution of phosphotungstic acid to which glacial acetic acid has been added? | Gomori One Step Trichrome Stain. |
| This stain is overstained (regressive) with a soluble lake of hematoxylin-ferric chloride iodine? | Verhoeff Elastic Stain |
| The principle for this stain is that Hydrochloric acid and paraldehyde are added to an alchoholic solution of basic fuchsin to form aldehyde fuchsin? | Aldehyde Fuchsin Elastic Stain |
| The principle of this stain is that the amount of phosphotungstic acid in the staining solutiin is far greater than the amount of hematein and it is believed that tungsten binds all available hematein to give a blue colored lake? | Mallory PTAH |
| The principle for this stain is the hexose sugars of reticulin are demonstrated by the oxidation to aldehydes? | Gomori Stain for Reticular Fibers |
| Silver ions from the methenamine silver compklex are first bound to the carbohydrate components of the basement membrane then reduced to visable metallic silver by the aldehyde groups? | Periodic Acid Methenamine Silver |
| Staining with oil soluble dyes is based on the greater solubility of the dye in the lipoid substances than in the usual hydroalcoholic dye solvents is the principle for this stain? | Oil Red O |
| The principle for this stain is the dye is slightly basic and will combine with acidic groups in compound lipids such as phospholipids? | Sudan Black B |
| This stain is a metachromatic dye and will stain a different color from the dye solution and the rest of the tissue? | Toluidine Blue for Mast Cells |
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