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MUA M2B2 GD
genetics block 2
Term | Definition |
---|---|
1. Coding regions | exons |
2. Non-coding regions | introns |
3. RNA polymerase Sigma factor = | Enables RNA polymerase to recognize and attach more tightly to promoter regions |
4. Translation | mRNA translated to Protein |
5. Rho-dependent termination | the RNA contains a binding site for a protein called Rho factor where the protein binds and moves towards the RNA polymerase |
6. Rho-independent termination | a region rich in C and G nucleotides which bind together and make a stable hairpin causing polymerase to stall. |
7. Actinomycin D | binds to the DNA template and interferes with the movement of RNA polymerase. |
8. Rifampin | Inhibits Prokaryotic RNA synthesis |
9. In eukaryotes mRNA is _____sistronic | monocistronic |
10. Promoter cites of RNA polymerase II | CAAT box, TATA box |
11. RNA polymerase II binds to | Transcription Factor IID on the TATA box. |
12. Pre-mRNA splicing is done by | snRNPs in a Spliceosome |
13. Alternative spicing examples | IMMUNOGLOBULINS, DOPAMINE RECEPTORS, TROPOMYOSIN VARIANTS |
14. Inhibitor of RNA polymerase II found in poisonous mushroom | α-amanitin-a in Amanita phalloides. Aka Death Cap |
15. α-Amanitin forms a tight complex with | polymerase, thereby inhibiting mRNA synthesis. |
16. Alpha – Amanitin treatment | -Gastrointestinal decontamination -high dose penicillin (inhibits amanitin in liver) -LIVER TRANSPLANT |
17. Terminating Codon | (UAA, UAG & UGA) |
18. Initiating Codon | (AUG) |
19. Protein coding region of mRNA is composed of a contineous, non-overlapping string of codons called | Open reading frame (ORF). |
20. Prokaryotic ORFs contain short sequences, (3-9bp) upstream of start codon called Ribosome binding site(RBS) or | Shine Dalgarno Sequence composed of 5’-AGGAGG-3’ which binds to the 5’-CCUCCU-3’ |
21. 3 modifications for the recruitment of ribosome to mRNA | Methylated guanine cap at the 5’ end , Kozak sequences :- presence of a purine 3 bp upstream of AUG and presence of a guanine immediately downstream. Presence of a poly A tail |
22. tRNA Terminus or acceptor arm | is 5’-CCA-3’ which is the binding site of amino acid to tRNA |
23. tRNA unusual or modified bases | uridine, thymine, pseudouridine, Inosine, dihydroxyuridine etc. |
24. Ribosome Large subunit | contains the peptidyl transferase |
25. Ribosome Small subunit | contains the decoding center |
26. Prokaryotic Ribosome | 50s+30s = 70s |
27. Eukaryotic Ribosome | 60s + 40 = 80s |
28. 16 S rRNA in the small subunit of ribosomes is | the binding site for the mRNA. |
29. 23 S rRNA of the large subunit is the | ribozyme which catalyzes the peptidyl transferase reaction. |
30. Translation proceeds in four phases | Activation, initiation, Elongation, Termination |
31. eLF are only in | eukaryotes |
32. Translation factors in eukaryotes that use GTP | eLF3, EF, EF2 |
33. Aminoglycosides | Binds 30 S ribosomal subunit and inhibit shine delgarno sequence |
34. Tetracyclines | Binds 30 S ribosomal subunit on inhibits elongation |
35. Chloramphenicol | Binds 50 S subunit and inhibits peptidyl transferase enzyme |
36. Macrolides | Binds 50 S subunit inhibits translocation of ribosome |
37. Linezolid | Binds 50 S subunit and inhibits association with 30 S |
38. Pseudomonias and Diphtheria toxin is an enzyme that modifies | EF-2 by transferring an ADP-ribosyl group from NAD+ to the diphthamide of EF-2 and shuts down eukaryotic protein synthesis |
39. Shiga toxin | Inactivates eEF-1….inhibits elongation (Cuts 28 S rRNA in 60S subunit of eukaryotes) |
40. Lac operon Promoter | Binds RNA polymerase |
41. Lac operon Operator | Binds the lac repressor protein |
42. Lac operon CAP site | Binds the Catabolite Activator Protein (CAP) |
43. lacZ | Encodes beta-galactosidase, converts lactose into allolactose |
44. lacY | Encodes lactose permease protein required for transport of lactose and analogues |
45. lacA | Encodes transacetylase modifies lactose and analogues |
46. The lac operon inducer is | allolactose, an alternative form of lactose, binds the repressor |
47. Glucose high cAMP low, lactose low | repressor on transcription low |
48. Glucose high cAMP low lactose high | repressor removed transcription low |
49. Glucose low cAMP high, lactose high | repressor removed transcription high |
50. Histone Acetylation | acetyl groups are attached to lysines in histone tails dna activation |
51. DNA methylation, the addition of methyl groups to certain bases in DNA | (usually cytosine), is associated with reduced transcription |
52. Enhancers | cis-acting DNA sequences that increase the rate of initiation of transcription by RNA polymerase II |
53. Micro RNA | Effectively blocks production of protein |
54. Interfering RNA | protect against viruses and turn off genes quickly |
55. Dicer, a multisubunit complex, binds to | double-stranded RNA molecules and cleaves them into ~21-nucleotide molecules called small interfering RNAs (siRNAs). |
56. SiRNAs bind to target mRNA and mark it for RISC complex where it is unwound and degraded | |
57. Wobble | Variations in the 3rd position on the mRNA codon often encodes the same amino acid. |
58. Missense mutation diseases | Sickle cell anemia -Phenylketonuria-G6PD -Galactosemia |
59. Nonsense mutation diseases | Hemophilia- factor VIII deficiency |
60. Splice site mutation diseases include | -β-Thalassemia -Tay Sachs disease-Gaucher’s disease-LDL receptor mutation |
61. Frameshift mutation disease | Duchene muscular dystrophy |
62. Dynamic mutation | is caused by the expansion of trinucleotide repeats within the genome |
63. Trinucleotide repeat mutation diseases | Fragile X, Huntingtons, Myotonic Dystrophy |
64. Restriction endonucleases recognize and cut | palindromic sequences |
65. Host Bacteria uses 2 enzymes in defense | Methylase (methyltransferase) and Restriction endonuclease (restriction enzymes) |
66. Plasmid cloning vector | are small, circular, extrachromosomal DNA cloning limit of 100 to 10,000 base pairs or 0.1-10 kilobases (kb). |
67. Phage cloning vector | about 1000 times greater than that of the plasmid vector. They also have a larger cloning limit than plasmids, consisting of 8-25 kb. |
68. Bacterial Artificial Chromosomes (BAC) | can accept DNA inserts of around 300 kb. |
69. Yeast Artificial Chromosomes (YAC) | is a synthetic DNA that can accept large fragments of foreign DNA (particularly human DNA). |
70. In vivo DNA Amplification | cloning |
71. In vitro DNA amplification | PCR |
72. Common PCR DNA polymerases | Taq or Pfu DNA polymerase |
73. Northern blot can be used to determine which tissues express FMRl gene | in fragile X syndrome |
74. Western blot used for HIV infection determination | Immunoblots, no bands = negative |
75. Restriction Fragment Length Polymorphism | Abolish the cleavage site of the restriction enzyme, Create a new cleavage site for a restriction enzyme, Insertion or deletion within a region flanked by a restriction enzyme |
76. M phase inhibitor | Vinca alkaloids and taxols bind to tubulin preventing mitosis |
77. S phase inhibitor | Antimetabolites inhibit DNA synthesis |
78. Etoposides inhibit | S and G2 phase by topoisomerase II inhibition which increases DNA degradation |
79. Bleomycin causes | G2 inhibition by increased DNA strand breaks |
80. Homologous recombination happens during | the pachytene stage of Prophase I during meiosis |
81. Chiasmata (chiasma) are where | chromosomes touch each other and exchange genes (crossing over.) |
82. Non-disjuction is the failure of chromosome pairs to separate during meiosis stage 1 or meiosis stage 2 specifically in the | anaphase |
83. If non-disjuction occurs during 1st meiotic division | all sperm abnormal |
84. Downs | . Chr 21 |
85. Edwards | Chr 18 |
86. Patau | Chr 13 |
87. XO | Turners Syndrome |
88. XXY | Klinefelter Syndrome |
89. XXX | Triplo-X Syndrome |
90. XYY | Jacob Syndrome |
91. G1 regulator | cyclin D, cdk4,6 |
92. G1and S checkpoint regulator | cyclin E , cdk 2 |
93. S regulator | cyclin A , cdk 2 |
94. M phase regulators | cyclin B cdk 1 |
95. Spindle checkpoint | block cytokinesis if all fibers not attached to kinetochores |
96. P53 | checkpoint in G1 that causes apoptosis |
97. Li-Fraumeni Syndrome | refers to the inherited predisposition to develop cancers in many organs owing to germ-line mutations of p53. |
98. E2F is a transcription factor that | mediates growth-dependent activation of genes required to make the transition into and through S phase. |
99. Rb binds and inactivates | E2F under conditions of growth suppression |
100. Normally, MetHb formed in the RBCs reduced back to Fe2+ by MetHb reductase enzyme system | |
101. By 3 month age, almost all Hb is of adult type | 2β2 (Hb A) |
102. HbS Variant: Sickle cell Disease | Valine(V) replaces Glutamic acid (E) residue at position 6 in the beta chains of globin |
103. HbS can be detected by | southern blotting |
104. HbC Variant: Hemolytic Anemia | Mutation in β globin HBB, GLU6LYS |
105. Hb Hammersmith | HBB, PHE42SER, Ser is smaller, heme drops out of pocket, Heinz bodies, hemolysis and cyanosis |
106. Hb Hyde Park | HBB, HIS92TYR, causes Methemoglobin |