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MLT-RNA Viruses 2
Virology
| Question | Answer |
|---|---|
| What are the other two families of RNA viruses? | 1.Flaviviridae 2.Retroviridae |
| What are the 4 genus of Flaviviridae? | 1.Flavivirus 2.Pestivirus 3.Hepacivirus 4.Pegivirus |
| What are the 2 examples of Flavivirus? | 1.Dengue virus 2.Zika virue |
| Flavivirus is positive-sense single strand RNA virus, what is the group under baltimore classification system? | Group IV |
| What is the genomic structure of Flavivirus? | Linear, 9.7-12 kb |
| How many ORF of the Flavivirus genome? | 1 |
| 1 single polyprotein will be produced, then it will be ______ individual proteins? | 10 |
| What are the 10 individual proteins? | 1. 3 structural proteins 2. 7 non structural proteins |
| What are the 3 structural proteins? | C, prM and E protein |
| What are the 7 non structural proteins and the functions? | 1.NS1 (Involved in RNA replication and particle assembly) 2.NS2A, NS4B (RNA replication and immune evasion) 3.NS2B, NS4A (cofactor of NS3) 4.NS3 (major viral protease) 5.NS5 (RNA-dependent RNA polymerase; methyltransferase for 5’ methyl cap synthesis) |
| What is the Initial target for Dengue virus? | immature dendritic cells (DCs) in skin |
| What is the primary target for Zika virus? | Gas6-AXL tyrosine kinase receptor complex |
| What is the major vector for Zika virus? | Aedes albopictus, a mosquito |
| How to detect Zika virus? | RT-PCR targeting the NS2A gene |
| Can ELISA be used to detect Zika virus and target antigen is? | Yes, Viral NS1 antigen |
| Which 3 cell lines can be used co culture Zika virus? | 1.African green monkey (Vero) 2.Rhesus monkey kidney (LLC-MK2) 3.Aedesmosquito cell lines MOS61 |
| What is the Baltimore group of Retroviridae? | Baltimore Group VI (ssRNA-RT) |
| How many copies of ssRNA in Retroviridae genome and what is the size? | 2, each 7-10kbp |
| What are the 3 types of enzymes can be found in the virion of retroviruses? | 1.Reverse transcriptase 2.Integrase 3,Protease |
| Each copy of Retroviridae genome consists of 4 parts, what are they? | 1.Long terminal repeat sequence 2.gag, group-specific protein gene 3.pol, polymerase gene 4.env, Envelop gene |
| What is the example genus of Retroviridae? | Lentivirus |
| What are the example diseases caused by Lentivirus? | AIDS( HIV I and HIV II) |
| HIV II is limited to ___________ only? | Africa |
| What are the 4 subtype of HIV I? | 1.Group M 2.Group N 3.Group O 4.Group P |
| Which is the major subtype of HIV I? | Group M, causes 90% of the disease of around the world |
| For HIV I entry to cell, ______ with bind to CD4 receptors on human cells? | gp120 |
| After binding gp120 to CD4, chemokine co-receptor triggers conformational change in ______? | gp41 |
| What are the 2 results of the conformational change in gp41? | 1.Leading to fusion of viral and cellular membrane 2.Release of nucleocapsid into cytoplasm |
| Each day, how many new viral particles are produced? | ~10^10 |
| What are the 2 consequences of massive infection of T cells? | 1.T-helper cell depletion 2.Susceptible to opportunistic infections |
| In late clinical phase of HIV I infection, CD4 T cells fall below ________ cells/ul? | 200 |
| What is the screening test for HIV infection in adults? | Enzyme-linked immunoassay (EIA) to detect antibodies against HIV in patient plasma |
| What is the confirmatory test for HIV infection in adults? | Western Blot |
| What are the target proteins for the western blot? | gp120, gp160, gp41, p31, p24 |
| What is the minimum band number required in western blot to confirm the HIV infection? | 3 |
Created by:
kencho
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