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Session 2 Microbio5
Microbio -5- Sterilization and Disinfection
Question | Answer |
---|---|
the process of destroying ALL microbes, endospores, and viruses on an object | Sterilization |
the process of eliminating most or all pathogens on an object | disinfection |
type of chemicals used on inanimate objects/Fomites to get rid of microbes | disinfectants |
type of chemicals used on skin and body surfaces to get rid of microbes | antiseptics |
What are the factors that affect the type of antimicrobial procedure to use | type of organism present number of microorganisms initially present environmental conditions potential risk for infection composition of item to be cleared of microbes |
what are the common types of microorganisms we try and get rid of | bacterial endospores- bacillus and clostridium protozoan cysts and oocysts- killed by boiling mycobacterium- waxy cells resistant to chemicals pseudomonas- resistant to and may grow on many chemical disenfectants (nosocomial infections) Naked Viruses |
why does number of microorganism present matter in antimicrobial selection | more organisms initially present the harder it will be to kill them |
what environmental conditions can affect antimicrobial selection | PH of environment, Temperature, Presence of fats, oils, or organic substances these can interfere with ability of anti microbial to kill |
What does potential risk of infection have to do with selection on antimicrobial used | if an object is likely to cause infection such as a scalpel or needle it has to be sterile some instruments only contact mucus membranes other should only contact unbroken skin so each could have a different antimicrobial treatment |
what are the three levels of potential risk of infection | critical instruments semicritical instruments non-critical instruments |
What impact does composition of item to be disinfected have on antimicrobial choice | if item could be destroyed by antimicrobial you will have to use another option such as a heat critical fluid that would have to filtered rather than heated |
what are the common types of anti-microbial procedures | PHYSICAL- heat(moist and dry), filtration radiation (ionizing, uv, microwave) CHEMICAL- alcohols, aldehydes, etc PRESERVATION- temp, water content |
boiling for how long should kill most organism and what organisms would not be killed | 5 minutes of boiling should kill everything but endospores |
does pasteurization sterilize | no, in only removes the majority of pathogens |
what is the heat method that uses pressure, volume and temperature | pressurized steaming |
when moist heat is not appropriate what other heat can be used | dry heat, such as incineration, and dry heat ovens which require longer times and higher temps than moist heat |
When would you use filtration to get rid of microbes | when the solution or gas could be destroyed by heat or chemicals |
How does radiation work as an antimicrobial | ionizing radiation- causes direct biological and DNA damage. Endospores are resistant UV radiation- cause DNA damage |
how are antimicrobial chemicals classified list the types | according to their capacity to kill organisms sterilants, high-level disinfectants, intermediate level disinfectants, low-level disinfectants |
chemical that can kill all microorganisms, viruses, endospore, used on critical instruments | sterilants |
can kill all viruses and vegatative microorganisms but can't reliably kill endospores. Used to treat semi-critical instruments and are generally sterilants used for less time | High-level disinfectants |
chemicals that can kill all vegetative microorganisms, fungi and most viruses. will not kill endospores regardless of incubation time can be used on non critical instrumetns | intermediate level disinfectants |
can kill fungi and vegetative bacteria except mycobacteria and only enveloped viruses | low-level disinfectants |
60%-80% solutions that are effective for killing bacteria and fungi, denature proteins used as antiseptic and disinfectants | alcohols |
capable of destroying all forms of microbial life including endospores and viruses act through inactivation of DNA and RNA germicidal in 10 min sporocidal in 3-10hrs | aldehydes |
iodophors which act by causing oxidative damage used as antiseptic or disinfectant, and chlorine compounds act by oxidative damage, hypochlorus acid formed when chlorine is dissolved in water | halogen |
act by disrupting cellular compounds, used to disinfect non-critical surfaces, inactivated by organic matter, pseudomonads and gram negative bac can be resistant | quarternary ammonia compounds |
act by disrupting membrane and denaturing proteins used in germicidal soaps | phenolics |
gas that can kill all microorganisms including endospores and viruses. acts by alkylation of nucleic acids. commonly used as a sterilant, can be used on items that cannot withstand steam/heat explosive in pure form mixed with N2 and CO2 | ethylene oxide |
chemical used in vaporized form as a sterilant in manufacturing causes oxidative damage | vaporized hydrogen peroxide |
what other factors do you need to keep in mind when selecting an antimicrobial besides organism to be killed | toxicity of the chemical, presence of organic matter, compatability with surface will it destroy it like electronic equipment, will it leave a residue |
what is the goal of preservation | slowing the growth of microorganism on commercial products |
what methods are used in preservation | chemical preservatives low temp storage reducing available water drying foods |
What are the classification of microorganism and how resistant are they | endospores- most resistant pseudomonas-highly chemical resistant mycobacterium- very chemical resistant protozoan cysts and oocytes- relative sensativity Naked Viruses- highly chemical resistant |
what determines the selection of antimicrobial chemical | cost and availability storage and stability environmental risks |
what are the endospore producers and what level of disinfectant are you most likely going to have to use on surface contaminated by them | bacillus and clostridium sp. will need a sterilant since only sterilants kill endospores |