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MLT-Parasitology
Microbiology
Question | Answer |
---|---|
What is protozoa? | An informal term for single-celled eukaryotes, either free-living or parasitic |
What are the 4 groups of protozoa? | 1.Flagellates 2.Amoeba 3.Sporozoa 4.Ciliates |
Whats medically important in Flagellates? | 1.Trichomonas vaginalis - genitourinary infection 2.Giardia lamblia - gut infection 3.Trypanosomes - blood infection 4.Leishmania - tissue infection |
Whats medically important in Amoeba? | 1.Entamoeba histolytica |
Whats medically important in Sporazoa? | 1.Plasmodium 2.Toxoplasma |
Whats medically important in Ciliates? | 1.Balantidium |
What would be caused by Giardia lamblia? | 1.Diarrhoea 2.Acquired by contaminated water or food |
Whats the specimen for Giardia lambia? | Examine fresh stool for trophozoites, or cysts |
Whats the shape and no. of nuclei of Giardia lambia ? | Pear shaped trophozoite and Cyst has 4 nuclei |
What would be caused by Entamoba histolytica? | Amoeba dysentery(痢) |
How to differentiate Trophozoites and Cysts? | Trophozoites: inhibit in hosts organ, non infective Cysts: for transfection |
Whats the treatment for Entamoba histolytica? | metronidazole |
Whats the specimen for Entamoeba histolytica? | 1. Fresh stool:wet mounts and permanently stained (eg.trichrome) 2.Concentrates from fresh stool: wet mounts, with or without iodine stain, and Trichrome, but not useful for demonstrating trophozoites |
What are hosts of Sporozoa-Toxoplasma gondii? | 1. Definitive host: Cat 2:Intermediate hosts: Human + other domestic animals |
Whats the ways to identify Sporozoa-Toxoplasma gondii? | 1. Serology: Immunofluorescence technique to detect specific Ag 2.Molecular diagnosis to detect DNA |
What are the hosts of Sporozoa-Plasmodium? | 1.Definitive host: Anopheles mosquitos 2.Infected host: Human |
What is the route of infection for Sporozoa-Plasmodium? | 1.Sporogonic cycle in Anophele mosquitos 2.Exo-erythrocytic cycle in Human 3.Erythrocytic cycle in Human 4.Mosquitos gametes produced in blood 5.Mosquitos takes the gametes during blood meal |
What would be caused by Sporazoa-Plasmodium? | Malaria |
What are the examples of Sporazoa-Plasmodium? | 1.P. vivax 2.P. falciparum 3.P. malariae 4.P. ovale |
What is the way to identify malaria? | Examine blood film, look for the morphological characteristics of the plasmodia |
What are the anti-malaria drug? | 1.Chloroquine 2.Artemisinin 3,Doxycycline |
What is Helmiths? | A worm like parasite |
What are the type of helminths? | 1.Nematodes 2.Cestodes 3.Trematodes |
What are the examples for Nematodes? | 1.Enterobius(Pin worm) 2.Necator(Hook worm) 3.Ascaris 4.Trichuris |
What would be caused by pin worm? | itching around anus |
How to identify pin worm? | 1. identifying eggs from stool or anal mucosa using Cellotape strip 2.Examine under microscope 3.Clear oval eggs, flattened on one side (25 um x 50um) |
What is the example for Ascaris? | 1.Ascaris lumbricoides 2.Man is the only host 3.Diagnosis: eggs or adult worms in stool |
What is the example for Trichuris? | 1.Trichuris trichura: Whipworm 2.Host: man, monkeys 3.Eggs are oval and brown, thick shelled structures with translucent knobs at the ends 50um x 25um in size 4.produce 3000 to 10000 eggs daily |
What is host for Hookworm? | 1.Man |
What would be caused by hookworm? | 1.Iron deficiency anemia 2.symptoms; gastrointestinal & nutritional symptoms |
Which stage/form of hookworm can penetrate skin and cause transmission? | Filariaform larvae |
What are the examples for Cestodes? | 1.Taenia saginata 2.Taenia solium |
What is the Cestode morphology? | 1. Long, 0.02m to 10m (scolex/head, neck, segmented body) 2.Scolex has 4 muscular disks for attachment to the intestinal mucosa 3.neck has proglottids 4.Each proglottid is a hermaphroditic reproductive unit |
What is beef tapeworm ? | 1.Taenia saginata 2.4-6 m in length |
What is the host for beef tapeworm? | Cattle is the intermediate host |
What would beef tapeworm cause? | epigastric pain, diarrhoea , nausea, irritability |
How to identify beef tapeworm? | eggs or proglottids in stool |
What is pork tapeworm? | Taenia solium |
How to differentiate T. saginata from T. solium? | T. saginata has no hooks in scolex and its uterus has 15-20 branches. T. solium has hooks in the scolex and its uterus has 7-13 branches. |