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GRAM POS. BACILLI
The Lord will bless me! Aja!
Question | Answer |
---|---|
non spore forming gram pos bacilli | Corynebacterium and Listeria |
contains endotoxin | Listeria |
catalase (+) central/sub terminal not swollen | Bacillus ( aerobic at 25-30C) |
catalase (-) terminal swollen spores | Clostridium (aerotolerant) |
largest pathogenic bacteria | Bacillus anthracis |
bamboo fishing rod | B. anthracis |
boxcar (cylindrical central, sub terminal spores) | B. anthracis |
non hemolytic with medusa head or beaten egg white colonies which is encapsulated and penicillin sensitive | B. anthracis |
maybe medusa head but has a wide zone of beta hemolysis; Penicillin resistant | B. cereus |
String of pearl test (35C) | B. anthracis |
for infected animals ( gram pos.) | Ascoli Test |
inverted pine growth ; slow liquefaction on gelatin | B. anthracis |
implicated in food poisoning ; ground glass appearance | B. subtilis |
for encapsulated strains | Fluorescent Antibody Staining |
Woolsorter's disease | B. anthracis |
VF: gamma D glutamic acid ; from herbivores (sheep, goats and cattles) | B. anthracis |
fried rice endospores ; no treatment for food poisoning (resistant to beta lactam antibiotics) | B. cereus |
compponent of the normal flora which is widespread in soil | Clostridium |
tennis racket, or drumstick ; motile (also boxcar) | C. tetani |
motile ; only lipase (+) clostridium | C. botulinum |
double zone of hemolysis ; (+) Nagler's test using EYA | C. perfringens |
phenylethyl alcohol with sheep's blood agar, BHI-S | Egg Yolk Agar (EYA) |
excellent selective and differential media for C. dificile | Cycloserine Cefoxitin Fructose Agar (CCFA) |
Lockjaw (Trismus) ; Risus sardonicus | C. tetani |
inhibits release of GABA and glycine from nerve cells resulting to sustained muscle contraction | tetanospasmin |
from stored vegetables, canned goods, ziplock storage bags, smoked fish and FRESH HONEY | C. botulinum |
Infant botulism ; flaccid paralysis also food borne botulism | C. botulinum |
Gas gangerene ; Pig bell and hyperbaric O2 treatment | c. perfringens |
pseudomembranous colitis ; horse manure odor | C. dificile |
Target hemolysis : complete theta toxin | inner zone |
Target hemolysis: incomplete alpha toxin or lecithinase | outer zone |
streaked perpendicular to a known group B streptococcus ; identifies C. perfringens | Reverse CAMP Test |
(+) for reverse CAMP Test | arrowhead hemolysis |