Save
Busy. Please wait.
Log in with Clever
or
show password
Forgot Password?
Don't have an account?  Sign up 
Sign up using Clever
or
Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account. Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't Know
Remaining cards (0)
Know
0:00
edit
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Gram Staining

Microbiology

QuestionAnswer
What is the gram stain procedure based on? Gram positive bacterial retain a crystal violet-iodine complex through decolorization with alchol.
What is a mordant? a coordination complex that is used to set die, by forming an insoluable complex in gram-positive cells.
What happens if you skip the mordant? the mordant fixes the crystal violet to the cell wall of gram + bacteria
What is the primary stain in gram staining? crystal violet, which is put on the cells for 20 seconds
Why does one want to use gram staining? What is the convience? Gram staining is used to differentiate bacterial species into two large groups (Gram-positive and Gram-negative) based on the physical properties of their cell walls
What is the 1st step of gram staining? Make a slide of cell sample to be stained. Heat fix the sample to the slide by carefully passing the slide with a drop or small piece of sample on it through a Bunsen burner three times
Why choose gram staining? It yields results quicker than a culture, and is especially important when a patient has an infection would make an important difference in the patient's treatment and prognosis; examples are cerebrospinal fluid can be gram stained for meningitis.
What is the second step of gram staining? apply crystal violet for 20 seconds
What is the 3rd step of gram staining? Wash with water 2 seconds
What is the 4th step of gram staining? Apply gram's iodine for 1 minute
What is the 5th step of gram staining? Decolorize with alcohol for 10 to 20 seconds, or until solvent flows colorlessly
What is the 6th step? Wash with water for 2 seconds
What is the 7th step? Apply Safranin for 1 minute
What is the 8th step wash 2 seconds
What is the 9th step? blot dry with bibuous paper, and air dry
What happens is the smear is too thick? allows one to examine the individual cells; furthermore, the thickness can affect decolorization. Thick smears can entrap the primary stain, which will not be removed by alcohol or acetone. Therefore, gram negative cells can appear to be a gram +
What is the most critical step in gram staining? Decolorization Why? If the destaining reagent is over applied, the dye-mordant complext can be removed from gram + cells, making them appear gram negative
What can you mess up with gram staining? need properly aged cultures 16 to 18 hours, if you leave on the alchol or acetone too long gram positive can appear gram - or gram varible
What else can you mess up? need thin smears, clumps will allow gram - to hold onto the crystal violet, thick smears will also not allow for leaching, so that you can tell the difference between gram - and gram +
What is the most crytical step? decolorization, do not flood with alchol or acetone during the decolorization process
Created by: stags
 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!
"Know" box contains:
Time elapsed:
Retries:
restart all cards