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C. Diff
Question | Answer |
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What are the agents incorporated in agar to recover C. diff for a stool culture? | Cycloserie, cefoxitin, fructose, egg yolk agar. Will appear as mustard-yellow colonies. |
What is the single most important risk factor regarding C. diff? | Antibiotic exposure because it alters the gut flora and allows this opportunistic pathogen to grow. Clindamycin has been identified as the main culprit. |
What is the principle of the TOX A/B Test and what should a positive result look like? | The TOX A/B Test is an enzyme immunoassay (EIA) for the detection of toxins A and B. A color change occurs due to the enzyme-antibody-antigen complexes that form in the presence of the toxins. A purple line indicates a positive result. |
Patients treated with antibiotics are often at risk for what? | Clostridium difficile associated diarrheal disease (CDAD), psedomembranous colitis (PMC), toxic megacolon, and death. |
What are some other tests for detection of C. diff? | Cytotoxicity Test/Tissue culture: looks at cell lines and presence of toxins. Latex Agglutination: recognizes the enzyme glutamate dehydrogenase. PCR, but is confined to research settings. Endoscopy. |
How is C. diff treated? | Once it is diagnosed, incited antibiotic must be discontinued if possible. Metronidazole is the first line therapy, and Vancomycin is the second line therapy. Fluid replacement is encouraged. |