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fix I
descriptions
| Question | Answer |
|---|---|
| Used to fix hematopoietic and lymphoreticular tissues? | B-5 |
| This type of fixative containes no water, is coagulating and nonadditive? | Nonaqueous Fixatives |
| This nonaqueous fixative is frequently used for touch preparations and blood smears ? | Methyl alcohol |
| Name the two reagents that are used as fixatives and are not compound fixatives. | Formaldehyde and Glutaraldehyde |
| Most fixative solutions are combined so that the disadvantage of one component will be ......? | counterbalanced by an advantage (or disadvantage) of another. |
| This fixative is known to give beautiful nuclear detail? | B-5 |
| List the three primary nonaqueous fixatives. | Acetone, Methyl Alcohol, Ethyl Alcohol |
| This solution is known for good nuclear preservation? | Bouin |
| Only used when the desired tissue components are destroyed or dissolved by aqueous fixatives? | Nonaqueous fixatives |
| This is used for enzyme studies, especially acid and alkaline phosphatase? | Acetone |
| Wash in iodine then sodium thiosulfate to remove mercury pigment before processing with this? | B-5 |
| Use this to preserve water soluble tissue components such as glycogen and urate crystals that are deposited in gout? | Ethyl alcohol |
| This is used for gastrointestinal and endocrine biopsies? | Bouin |
| The acetate present in this solution stabilizes RBC membranes and the granules of eosinophils and endocrine cells? | Hollande |
| Tissue must be treated for mercury pigment after immersion with this fixative. If excess fixative is not removed by washing with water, the formation of a chrome pigment can arise? | Zenker or Helly |
| This fixative is very stable and although not widely used, it is a good general purpose fixative? | Zamboni (Buffered Picric Acid-Formaldehyde or PAF) |
| Tissue cannot remain in this solution indefinitely; after fixation, wet tissue must be placed in a storage solution of 70% alcohol? | B-5 |
| This fixative is NOT used for EM studies or or nucleic acid demonstration? | Bouin |
| This type of fixative will overharden and shrink tissue? | Nonaqueous |
| What do you wash sections fixed with B-5 in to remove mercury pigment? | Iodine and then sodium thiosulfate |
| You can use this for fixation of brain tissue for a diagnosis of rabies? | Acetone |
| This fixative is good for immunohistochemistry and immunofluorescence and most special stains. NOT satisfactory for silver stains? | B-5 |
| Red blood cells are lysed with this fixative because of its acetic acid content. Iron and small calcium deposits are usually dissolved, and formalin pigment may be obtained. | Bouin |
| This alcoholic Bouin solution is excellent for the preservation of some carbohydrates, especially glycogen. As with Bouin solution, the excess picric acid should be removed by washing with 80% alcohol? | Gendre |
| This is an excellent fixative for the Trichrome stain? | Bouin |
| The swelling effect of acetic acid is balanced with the shrinking effect of picric acid, and the hardening effect of formaldehyde is counteracted by the soft fication of picric acid with this fixative? | Bouin |
| This fixative can be used in frozen sections for cell surface antigens by immunohistochemistry? | Acetone |
| This solution will lyse erythrocytes because of the acetic acid present and is good for performing Mallory PTAH? | Zenker |
| This fixative allows for secondary fixation with osmium? | Zamboni |
| This fixative preserves glycogen and is used in cytology for its ability to lyse erythrocytes? | Carnoy |
| This fixative is recommended as a postfixative after NBF. Antigenicity is enhanced, and nuclear detail is improved? | Alcoholic Zinc Formalin |
| Using this fixative, formalin pigment (acid hematin) can be produced. A minimum of 4 to 6 hours should be allowed for fixation of biopsy tissues and 6 to 8 hours for most other tissues. | Unbuffered Aqueous Zinc Formalin |
| This is ot a good general purpose fixative but is preferred for the demonstration of chromaffin (chromate-loving) granules in the cytoplasm of cells of the adrenal medulla? | Orth |
| This preserves structures with soft and delicate textures? | Bouin |
| This modification of Bouin solution is stable and will decalcify small specimans of bone. It is becoming widely used as a fixative for biopsy specimans if the gastrointestinal tract? | Hollande |
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