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ID Labs 2
Infectious Disease
Question | Answer |
---|---|
Indirect immunofluorescent antibody, Weil-Felix reaction, and complement are tests for: | RMSF (Rickettsia rickettsia) |
Histoplasmosis CBC | Organism in neutrophils & monocytes on Wright-Giemsa PBS; in macrophages on bone marrow smear |
Histoplasmosis labs | high alk phos, LDH, ferritin; anemia of chronic dz; pancytopenia |
Tests for Cryptococcus | cryptococcal Ag in CSF/serum; India ink or serology w/latex agglutination; CRAG |
RMSF labs | CSF: pleocytosis & hypoglycorrhachia. Low platelets & Na, high LFTs & bili. Serum IFA to confirm dx (may not see Ab response for 2 weeks) |
Clustered bacteria in vacuoles in WBCs; low platelets; leukopenia w/left shift; high LFTs; rising Ab immunofluorescence titer | Ehrlichiosis |
Histoplasmosis CBC | Organism in neutrophils & monocytes on Wright-Giemsa PBS; in macrophages on bone marrow smear |
Aspergillosis Dx test: | Bx of lesion |
Large septate hyphae that branch at a 45 degree angle: | Aspergillus (eg, A fumigatus) |
Organism grows thick-walled cells with single broad-based bud: | Blastomyces |
Histoplasmosis labs | Anemia of chronic dz. Pancytopenia, high alk phos, LDH, transferrin in disseminated dz. |
Sputum cx in Histoplasmosis is usually: | Negative |
Histoplasmosis labs in immunocompromised patients | BM & blood cx usually positive. AIDS pt with disseminated dz: urine antigen assay 90% sensitive |
Giardiasis labs | 3 stool specimens each 2 days apart: O&P for cyst & troph. Antigen test cheaper, less sensitive & will not detect other organisms |
Malaria labs | Thin & thick PBS Q8h x3 days for dx & TOC. Rapid antigen test. High then low WBC, high monocytes, possibly anemia & high LFTs |
CT or MRI showing ring-enhancing lesions in the brain (corticomedullary junction or basal ganglion) suggests: | Toxoplasmosis (often in immunocompromised pt) |
Thick white vaginal discharge, hyphae & buds on KOH prep | Candida |
Test of choice for MAC | Blood cultures (98% sensitivity) |
Hansen disease may see false positives in these tests | Syphilis, antithyroglobulin Ab, RF |
Tetanus labs | Moderate leukocytosis, normal CSF, normal Ca |
Lyme labs | ELISA (serum Ab) with western blot to confirm. Half of pts neg Ab in first few weeks |
Glanders labs | Serology preferred. 16s rRNA gene sequencing for rapid ID. |
Glanders on CXR | Military nodules, lung abscesses, lobar, or bronchopneumonia |
GNR demonstrating bipolar “safety pin” staining with Wright, Giemsa, or Wayson stain = | Yersinia pestis (grows best on blood agar or MacConkey at 35C) |
Q fever labs | Requires special reference lab testing. TOC is indirect immunofluorescence. Also ELISA & complement fixation. High RF, CRP, ESR +/- LFTs & WBC |
Smallpox labs | Testing at high-containment (BL-4) facility. Electron microscopy to confirm. |