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micro 3-26 lab gel
gel electrophoresis of dna samples
| Question | Answer |
|---|---|
| a technique that allows DNA fragments to be separated based on size and obseved with the naked eye | gel electrophoresis |
| DNA is loaded into wells or depression at one end of a gel composed of ___ | agarose (purified agar) |
| DNA will move toward the positive pole aka an | anode |
| DNA fragments subjected to electrophoresis will separate into distinct __ of different size categories | bands |
| DNA prepared for electrophoresis are combined with __ | tracking dyes |
| the 2 types of dye used as tracking dyes are __ and __ | xylene cyanol and bromphenol blue |
| ___ migrates at a rate similar to 3000-4000bp DNA fragments | xylene cyanol |
| __ migrates at a rate similar to 100-300pb | bromphenol blue |
| a chemical that produces an orange fluorescene when in UV light | ethidiym bromide |
| ethidium bromide stained gels will be placed over a UV light called | transilluminatior |