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Unit 4 Chapter 20
Vibrio , Aeromonas , Plesiomonas and Campylobacter Species
Question | Answer |
---|---|
Vibrio *colony morphology* | polar, sheathed flagella, curved, highly polymorphic |
Vibrio *gram stain results* | gram- negative rods |
Vibrio *testing results* | oxidase-positive, catalase-positive, reduces nitrate to nitrites, positive mucoid stringing |
Vibrio *virulence factors* | common flagellar, H antigens, somatic o antigens |
Vibrio *epidemiology* | aquatic environments including fresh water, brackish or esturine water, and marine or salt water. |
Vibrio *infections* | gastroenteritis, cholera, fatal septicemia, necrotizing fasciitis |
V cholera *colony morphology* | medium to large smooth opaque, iridescent with greenish hue, α or β- hemolytic |
V cholera *gram stain results* | gram-negative curved rods |
V cholera *testing results* | VP positive, Hemolyzes, erythrocytes, inhibited by polymyxin B |
V cholera *virulence factors* | resistance to tetracycline, doxycycline |
V cholera *epidemiology* | developing countries, Bengal, Bangladesh, asia and Africa, central America |
V cholera *infections* | cholera, gastroenteritis, motile, single polar flagellum |
Aeromonas *colony morphology* | mesophilic or psychrophilic, large round raised opaque, smooth and often mucoid |
Aeromonas *gram stain results* | Gram negative rods |
Aeromonas *testing results* | Oxidase positive, Glucose Fermenting |
Aeromonas *virulence factors* | multifactorial patterns associated with enteric disease |
Aeromonas *epidemiology* | fresh water, estuarine. Marine environments |
Aeromonas *infections* | pediatric diarrhea, gastrointestinal infection, inflammatory bowel disease, extra intestinal infections |
Plesiomonas *colony morphology* | Motile by two to five lophotrichous polar flagella; swarming does not occur; Colonies appear grayish, shiny, opaque with a smooth surface. |
Plesiomonas *gram stain results* | Gram-negative. Round ended, straight rods |
Plesiomonas *testing results* | Oxidase-positive. Catalase-positive. Lysine-, Ornithine-, and Arginine-Decarboxylase-positive. Indole-positive. Lipase-negative. Most strains are sensitive to vibriostatic agent 0/129. DNase-negative. Voges-Proskauer-negative. |
Plesiomonas *virulence factors* | resistance to penicillin class of antibiotics and more than one amnioglycoside. |
Plesiomonas *epidemiology* | soil and aquatic environments; widely distributed among warm- and cold-blooded animals |
Plesiomonas *infections* | gastroenteritis, extra intestinal infection |
Campylobacter *colony morphology* | curved, non-spore-forming, moist runny looking and spreading, usually nonhemolytic, round raised or flat; can produce smooth convex translucent colonies. |
Campylobacter *gram stain results* | gram-negative rods |
Campylobacter *testing results* | jejuni: catalsae-positive, reduces nitrate to nitrite, urease- negative, H2S - negative, hippurate hydrolysis -positive, Indoxyl acetate hydrolysis - positive |
Campylobacter *virulence factors* | multiple flagella |
Campylobacter *epidemiology* | direct contact with infected pets, indirectly by consumption of contaminated water, dairy products, and improperly cooked poultry, some spp. are sexually transmitted |
Campylobacter *infections* | diarrheal disease, nausea, vomitting |
Helicobacter pylori *colony morphology* | convex and translucent in appearance, slight hemolysis around colonies, which are grey in color. |
Helicobacter pylori *gram stain results* | Gram-negative, microaerophilic |
Helicobacter pylori *testing results* | catalase - positive, variable nitrate reduction, urease - negative, TSI - negative, Hippurate Hydrolysis - negative, Indoxyl Acetate Hydrolysis - negative |
Helicobacter pylori *virulence factors* | strong ureolytic ability, sheathed flagella |
Helicobacter pylori *epidemiology* | gastric or intestinal sites in mammalian host animals. |
Helicobacter pylori *infections* | gastric, peptic. and duodenal ulcers as well as gastrointestinal carcinoma |