click below
click below
Normal Size Small Size show me how
Unit 1 Chapter 8
Use of Colonial Morphology for the Presumptive Identification of Microorganisms
Question | Answer |
---|---|
Define plate reading | interpretation of Gram-stained smears from clinical specimens and microbial colonies. Provides initial identification of microorganism by microscopic characterization, description of physical growth characteristics of microorganisms on laboratory medium |
Define hemolysis | dissolution or breaking apart pertaining to red blood cells; a reaction, especially caused by enzymatic or toxin activity of bacteria, observed in the medium immediately surrounding or underneath the colony. |
List and explain all types of hemolysis | α-Hemolysis- is a partial lysing of erythrocytes around an under the colony that results in a green discoloration β-Hemolysis- is a complete clearing of erythrocytes around or under the colonies because of the because of the complete lysis of RBCs. |
Define colony morphology and list descriptive terms used and explain or draw them | colony characteristics and form. size form or margin elevation density color consistency pigment odor |
Define pigment and give examples | coloring matter or substance: green, sometimes metallic (P. aeruginosa) brick-red (Serratia marcescens) blue (Kluyvera spp.) purple ( Chromobacterium violaceum) brown-black (Prevotella melaninogenica) |
Define odor and give examples | the odor is determined when the lid of the culture plate is removed and it dissipates into the surrounding area old socks(S.aureus); grapelike(P.aeruginosa); putrid(P.mirabilis); mousy(Haemophilus spp); freshly plowed field (Nocardia spp) |