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True or False

Translation True or False 2

QuestionAnswer
True or False: In eukaryotes transcription takes place in the cytoplasm and translation takes place in the nucleus; the two processes are uncoupled. False. In eukaryotes transcription takes place in the nucleus and translation takes place in the cytoplasm; the two processes are uncoupled.
True or False: The -35 and -10 region represent binding sites for RNApol holoenzyme and initiation of transcription. True
True or False: The -35 and -10 region represent binding sites for the Pridbnow box and initiation of transcription. False. The -35 and -10 region represent binding sites for RNApol holoenzyme and initiation of transcription.
True or False: The -10 sequence in bacteria is CG-rich, making the strands easier to separate so the transcription bubble can be formed at this site. False. The -10 sequence in bacteria is AT-rich, making the strands easier to separate so the transcription bubble can be formed at this site.
True or False: The -10 sequence in bacteria is AT-rich, making the strands easier to separate so the transcription bubble can be formed at this site. True
True or False: The function of the sigma factor in RNApol holoenzyme is to bind the promoter sequences. True
True or False: The function of the sigma factor in RNApol holoenzyme is to find the promoter sequences. False. The function of the sigma factor in RNApol hlolenzyme is to BIND the promoter sequences
True or False: In the absence of the sigma factor, RNApol specifically binds to the promoter and begins transcription. False. In the absence of the sigma factor, RNApol binds to all DNA equally well; binding of the sigma factor is required for initiation of transcription.
True or False: In the absence of the sigma factor, RNApol binds to all DNA equally well. True
True or False: In eukaryotic transcription, in the absence of the sigma factor, RNApol specifically binds to the promoter and begins transcription. False. The sigma factor has nothing to do with eukaryotic transcription.
True or False: In eukaryotic transcription, in the absence of the sigma factor, RNApol binds to all DNA equally well. False. The sigma factor has nothing to do with eukaryotic transcription.
True or False: An Mg++ in the active site of DNA polymerase enhances the nucleophilic attack by the 3'OH on the incoming dNTP. True
True or False: An Fe++ in the active site of DNA polymerase enhances the nucleophilic attack by the 3'OH on the incoming dNTP. False. An Mg++ in the active site of DNA polymerase enhances the nucleophilic attack by the 3'OH on the incoming dNTP.
True or False: DNA polymerase adds a dNTP to the 5' end of a growing DNA strand. False. DNA polymerase adds a dNTP to the 3' end of a growing DNA strand.
True or False: DNA polymerase always begins the synthesis of a DNA strand with a ribonucleotide. False
True or False: Both sythesis and proofreading activities are catalyzed by a pair of Asp residues in the active site of the DNA polymerase. False.
True or False: The biochemical function of helicase is to separate the strands at the replication fork. True
True or False: The sliding clamp (PCNA) is required to separate the strands at the replication fork. False. PCNA is required for processivity; it enables DNApol to copy long stretches of DNA without falling off the template.
True or False: The biochemical function of primase is to synthesize a copy of RNA to start a new DNA strand. True
True or False: The biochemical function of primase is to synthesize a copy of DNA to start a new DNA strand. False. The biochemical function of primase is to synthesize a copy of RNA to start a new DNA strand.
True or False: Telomerase is unique because it does not require a template since it contains an RNA that it uses instead. True
True or False: Telomerase is requires a template to being synthesis of the telomer. False. Telomerase is unique because it does not require a template since it contains an RNA that it uses instead.
True or False: Teomerase is unique because it is the DNA polymerase responsible for mitochondrial replication. False. Telomerase is unique because it does not require a template since it contains an RNA that it uses instead.
True or False: The most important mechanism for determining the start site of transcription in bacteria is the binding of sigma factor to -10 and -35 sequences. True
True or False: The most important mechanism for determining the start site of transcription in bacteria is recognition of the Shine-Dalgano sequence by a spcific binding protein. False. The most important mechanism for determining the start site of transcription in bacteria is the binding of sigma factor to -10 and -35 sequences.
True or False: The most important mechanism for determining the start site of transcription in bacteria is recognition of the transcription origin by dnaA. False. The most important mechanism for determining the start site of transcription in bacteria is the binding of sigma factor to -10 and -35 sequences.
True or False: The function of Rho protein in bacterial transcription is to put the 5' cap on the mRNA. False. The function of Rho protein in bacterial transcription is to bind a sequence at the 3' end of the mRNA to terminate transcription.
True or False: Rho is the RNA polymerase responsible for transcribing ribosomal RNA in bacteria. False. Rho binds a sequence at the 3' end of the mRNA to terminate transcription.
True or False: Rho binds to specific sequences in the promoter to initiate transcription. False. Rho binds a sequence at the 3' end of the mRNA to terminate transcription.
True or False: Rho binds a sequence at the 3' end of the mRNA to terminate transcription. True
True or False: Footprinting is a biochemical technique that is used to identify posttranscriptional modifications of bases in RNA. False. Footprinting is a biochemical technique that is used to identify where a DNA binding protein binds to a DNA molecule.
True or False: Footprinting is a biochemical technique that is used to identify the location of splice sites in RNA. False. Footprinting is a biochemical technique that is used to identify where a DNA binding protein binds to a DNA molecule.
True or False: Footprinting is a biochemical technique that is used to identify the location of stem-loop structures in RNA. False. Footprinting is a biochemical technique that is used to identify where a DNA binding protein binds to a DNA molecule.
True or False: Footprinting is a biochemical technique that is used to identify where a DNA binding protein binds to a DNA molecule. True
True or False: RNA pol II is recruited to the transcription initiation site by the TFII complex. False
True or False: The C-terminal domain of RNA pol II has Ser residues for phosphorylation which are essential for transcription. False
True or False: RNA pol II is responsible for the transcription of both mRNA and tRNA. True
True or False: RNA pol II requires Mg2+ for catalytic activity. False
Created by: P1StudyStack
 

 



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