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Micro Water

Micro106 - Microbiology of Water

QuestionAnswer
What do typhoid fever, cholera and dysentery have in common? They are waterborne diseases caused by enteric bacteria (coliforms).
What is a coliform? A coliform is a gram negative, falcultative anaerobic, non-spore forming rod that ferments lactose to acid & gas.
What does the presence of E. coli in water mean? E. coli in water provides evidence that fecal contamination has occurred.
What is a presumptive test? 15 lactose broth tubes with durham tubes are inoculated with water and observed for gas production. Gas production occurs, it is presumed to contain coliforms.
What does MPN stand for? MPN stand for most probable number of coliforms per 100 ml of water.
What is a confirmed test? Positive cultures from the presumed test are streaked onto selective & differential media to see if the gas producing organisms are gram negative.
What agar is used in the confirmed test? Levine EMB agar is used. It includes two dyes, eosin & methylene blue that inhibit the growth of gram positive bacteria.
What will positive results look like from a confirmed test? Lactose fermenting bacteria are uniquely pigmented, E coli will appear blue-black with a green metallic sheen.
What is a completed test? A Gram stain is done using a representative colony from the confirmed test.
Created by: jrb265
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