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VA Microbiology
VTA 130 Microbiology
Question | Answer |
---|---|
list the three basic shapes of bacteria | coccus (round), Bacillus (rods), spirillum (spirals) |
Gram negative bacteria stain is what color | pink |
Gram positive bacteria stain is what color | purple |
what is the purpose of a gram stain | to differentiate between gram positive and gram negative bacteria |
what are the 3 pieces of information we label on a culture plate | client name, date, where swab came from |
how is a culture plate placed in the incubator | upside down |
what are the three types of hemolysis | alpha, beta, gamma |
name two types of stain used in microbiology | New Methylene blue, Gram |
define morphology | the description of size, shape, color |
what is the scientific name for canine ear yeast | Malassezia pachydermatis |
what is DTM | drematified testing media |
describe alpha hemolysis | green/brown incomplete hemolysis |
describe beta hemolysis | complete hemolysis |
describe gama hemolysis | no color, no hemolysis |
what are the Gram stains used in order (CIDS) | crystal violet, iodine, decolorize, safranin |
what is the first thing you do in the Gram stain procedure | heat fix |
what are the types of media | basic, enriched, selective, differential, Mueller-Hinton Agar, |
what is enriched media enriched with | 5% sheep's blood |
what is hemolysis | blood destruction |
what is the first lab rule | assume all cultures are bio hazardous waste |
name the three basic shapes of bacteria | cocci (round), bacillus (rods), sprillium (spiral) |
what do we do use a gram stain for | it is the first step in identifying a bacterial organism or pathogen |
what is the first step we do when doing a gram stain | heat fix it |
what are the stains we use in a gram stain | crystal violet, iodine, decolorer, safranin |
how long do we allow the decolorer stain to stay on | 10 sec |
why do we do a gram stain | to differentiate between gram positive and gram negative bacteria |
what is formites | inanament objects that transfer bacteria |
what is nosocomial | acquiring bacteria from a health care facility |
what is an aerobic specimen | requires air to live |
what is an anaerobic specimen | can't survive in oxygen |
what are the six types of media | basic, enriched, selective, differential, Mueller-Hinton agar, broth |
what is hemolysis | blood destruction |
what is morphology | size, shape, color, etc |
what is the BAP | blood auger plate |
what is the enriched media enriched with | 5% sheeps blood |
what is DTM | dramatophyte testing media |
What temp is the BAP incubated at | 37 degrees C = 98.6 degrees F/ body temp |
what is a DTM incubated at | room temperature |
to inoculate our BAP plate we need what | gloves, sterile swab, media, incubator |
what do we use the Mueller-Hinton auger used for | for antibiotic susceptibility testing |
what will grow lime green under the woods lamp | ring worm |
what is a drematafite | ring worm |
what is mycology | study of fungus |
what does canine ear yeast look like under the microscope | like a footprint or a kidney bean |