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AGT Lab Manual Information

Where to find spontaneously dividing cells bone marrow, lymph node, testis, chorionic villi of placenta, some leukemic blood, solid tumor, some pleural or ascetic fluid, fetal ascites or cystic hygroma, and often fetal or newborn blood; plus blood lymphocytes, amniotic fluid, skin and fibroblasts
Mitotic arresting agents Colcemid, colchicine, or Velban
Who coined the words mitosis, chromatin, prophase, metaphase, anaphase, and telophase Walther Flemming, 1879-1889
Who coined the word chromosome in 1888 W. Waldeyer
Q banding method discoverer Caspersson in 1968: quinacrine mustard stain; allowed identification of each pair as well as small parts of each chromosome
Tissue mosaic syndromes Pallister-Killian syndrome and trisomy 20 mosaicism
Suspension Culture tissue types bone marrow, blood, some neuroblastomas, and most lymphomas, suspended in growth medium and do not attach to the culture vessel
Attached monolayer culture tissue types amniocytes, skin fibroblasts, which require adherence to the culture vessel to grow
Fanconi's anemia open system gas exchange no oxygen and may be gassed only with nitrogen (sensitive to the presence of oxygen)
Media with buffering systems CO2-bicarbonate equilibrium and HEPES buffer
RPMI 1640 type of culture Blood, bone marrow, solid tumor
RPMI 1603 type of culture Blood, bone marrow
Media for all types of culture MEM or Eagle's MEM (minimal essential medium); MEM Alpha; DME (Dulbecco's modified Eagle's); Ham's F10; Ham's F12; McCoy's 5A
Fragile X media Medium 199 or TC 199 (low folic acid media)
Media for Amnio and CVS Chang; Amniomax; Amniogrow (all for neoplastic cells)
Relative centrifugal force calculation RCF = 0.00001118 x r(radius in cm) x Nsquared (rotating speed, RPM)
Hypotonic affects: spreading, chromatid width and separation, and in bloods and bone marrows, the elimination of red blood cells
Colcemid concentration affects: chromosome spreading and morphology
Increased dosage of Colcemid: increases MI, straightens chromosomes, crisps chromatid edges, and increases chromosome spreading as it releases them from the mitotic apparatus
colchicine, a derivative of autumn crocus
Velban Vinblastine sulfate, mitotic arrestant
Created by: MorganRiso

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