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UND 363 Alizarin Red
UND 363 Alizarin Red S Calcium
Question | Answer |
---|---|
purpose for Alizarin Red S | id of calcium in tissue |
principle | calcium forms an alizarin complex via a chelation process |
fix | alcoholic formalin or NBF (acidic fixes and pretreatments must be avoided) |
tech | 4-5 and FS |
qc | section with calcium |
reagents | alizarin red s/ammonioum hydroxide - chelation (ph must be 4.1-4.3 CRITICAL), acetone - dehydration, acetone/xylene - dehydration |
what is used to adjust the ph of this stain | .5% ammoniom hydroxide |
results | calcium - orange red |
what are 2 importants facts about this stain | reaction product is birefringent, and the reaction must be controlled microscopically to prevent diffusion artifact |
what does the dahl version of this use in addition to the reagents | lt green counterstain |