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UND 363 Gomor urates
UND 363 Gomori Methanimine silver urates
Question | Answer |
---|---|
purpose for Gomori Methenamine silver | demo urates. unstable uric acid metabolism (gout) caused deposits of urate crystals around joints and in soft tissue |
what are the collections of urate crystals called | gouty tophi |
principle for Gomori Methenamine silver | urates stained black (presumed via silver reaction) and then reduced to metallic form |
fix | ABSOLUTE ALCOHOL REQUIRED |
tech | 4-5 and chem cleaned glass |
qc for Gomori Methenamine silver | section with urates |
reagents | silver nitrate/hexamethylenetetramine/sodium borate - impregnation, sodium thiosulfate - remove unreduced silver, lt green - counterstain |
results | urates - black, background - blue green |
what can also be demoe inadvertantly with this tech. | calcium, if the deposites are large enough to withstand dissolving in silver stain soln' |