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UND 363Turnbull Blue
UND 363 Turnbull Blue
Question | Answer |
---|---|
purpose for Turnbull Blue | demo FERROUS (Fe2+) iron |
why is ferrous iron not normally stored in tissue | it is toxic, if it absorbed by intestine but is rapidly converted to ferric form |
principle for Turnbull Blue | sections treated with acidic potassium FERRICcyanide, ferrous iron will form insoluble bright blue pigment (turnbull blue ie ferrous ferricyanide) |
fix for Turnbull Blue | alcohol or NBF |
techfor Turnbull Blue | 4-5 and chem cleaned glass |
qc | section with FERROUS iron |
reagents in order | hcl/potassium FERRICcyanide mix -ferrous ions react to form ferrous ferrocyanide, acetic acid - rinse, nuclear fast red - counterstain |
results | ferrous iron - blue, background - pink/red |