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UND 363 Dieterle
Question | Answer |
---|---|
purpose for Deiterle method | demo spirochetes or causitive organisms of legionella |
principle | argyrophil method (ability to bind or be impregnated with silver ions but require reducing agent) - spirochetes can bind silver but need hydroquinone to reduce to visible |
fix | NBF |
micron | 4-5 |
QC | tissue with spirochetes or Legionella, chemically clean glassware, non metallic forceps |
reagents in order for Deiterle method | alcholic uranyl nitrate - sensitizer, alcoholic gum mastic - pretreatment, Silver nitrate - impregnation, Developer (hydroquinone/soduim sulfite/acetone/formaldehyde/pyridine/alcoholic gum mastic, Formic Acid |
what is the purpose of the formic acid in this stain | it is unknown |
what caution must be use with the 1st step alsoholic uranyl nitrate | temp cannot exceed 60○C or the silver will not precipitate |
results for Deiterle method | spirochetes/bacteria - brown or black, background - pale yellow or tan, other structures (melanin, chromatin, formalin pigment, pigmented macrphages) - brown to black |