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UND 363 Biel M'wave
UND 363 Bielschowsky Mwave
Question | Answer |
---|---|
purpose for biel m'wave | demo nerve fibers and neurofirillary tangles/senile plaques |
principle | impregnation with ammoniacal silver, recudtion from formaldehyde, NOT Toned w/ GOLD, sodium thiosulfate for excess silver removal |
what will occur since gold chloride is not used in this method | the background will remain yellow. |
fix | formalin |
tech | 8 micron |
qc | CNS must be used (tissue should contain senile plaques and neurofibrillary tangles from alzheimer pt. if possible) |
results | axon/cytoplasmic neurofibrils - brown to black, neurofibrillary tangles and plaques (from alzheimer pt's) - dk brown or black, neromelanin - black, lipofuscin - brown or black, blackground - yellow |
notes | must use chem cleaned glassware that is rinsed twice in distilled water. |
what is positive about this stain method | requires much less silver nitrate |