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UND 363 Tricrome Gen
UND 363 Trichrome General and collage/muscle techs.
Question | Answer |
---|---|
what can trichrome stain differientate | collagen from smooth muscle (can also be used for fibrin and RBC's) |
what are the dyes specific for | one is a nuclear dye and the other two are acid dyes of different color (for selectively coloring basic tissue) |
what would be a clinical application for the trichrome stain | and increase in collage will indicate fibrotic change (ie cirrhosis of liver), also good for determining neuromuscular disease and tumors of muscle tissue |
what is better used than the trichrome stain | IHC (can determine specific cell types), but trichrome still good |
what are 5 factors that affect the stain | tissue permeability and dye molecule size, heat, ph, nuclear stain, fixation. |
how does molecule size affect | smaller molecules are better, but if a larger one will fit it will replace the smaller one |
how does heat affect stain | heat influences rate of staining and penetration of the dye |
how does PH affect stain | (1.5 - 3.0 for trichrome), ph can affect nuclear stain (ex alum. hematox will decolor in adic soln') |
how does the nuclear stain affect the trichrome | weigerts used because it is an iron hematox (is more resistant to acid soln' Vs. alum hematox) |
how does fixation affect stain | prolonged formalin will cause several tissue groups to be saturated, therefore fewer groups to react with trichrome stain |
How can the problem of prolonged fixation be remedied | tissue can be treated (mordanted/post fix) with picric acid, bouins, or mercuric chloride soln' |
what is the difference between the multistep ad one step technique | multi - dyes applied one after another and optimized at each step, One step - dyes and reagents combined - the reactions occur simultaneously (ie van gieson/gomoris |
give 4 stain techniques for collagen and muscle | van gieson (acid aniline dye w/picric acid), masson tri (phosphomylbdic and phoshpotungstic acid), PTAH, Periodic acid oxidation method. |