click below
click below
Normal Size Small Size show me how
UND 363 MM Coll FE
UND 363 Muller-mowry colloidal Iron
Question | Answer |
---|---|
what is M.M. Colloidal Iron often used instead of? | alcian blue PAS |
purpose of M.M. Colloidal Iron | demonstrate carboxylated and sulfated mucopolysaccharides and glycoproteins |
what is the ADV. of M.M. Colloidal Iron over alcian blue PAS | more sensitive for acid mucins particularily when they are in small amounts (ie acid mucins in mesotheliomas) |
principle of M.M. Colloidal Iron | colloidal ferric iron ions absorbed by carboxylated and sulfated mucosubstances because of low PH **iron bound tissue will be shown by the prussian blue reaction** |
Explain how the prussian blue reaction forms | potassium ferrocyanide treatment- the ferric ions will form ferric ferrocyanide (ie prussian blue) |
general fixatives of M.M. Colloidal Iron | 10% NBF, Carnoys, alcoholic formalin AVOID chromatic fixatives (will oxidize polysaccharides ie Zenkers, Hellys, orths) |
thickness/control for M.M. Colloidal Iron | 4-5micron, sm. bowel, appendix, colon |
microscope for M.M. Colloidal Iron | Light |
list the 5 major reagents of M.M. Colloidal Iron | Acetic acid *12%*, colloidal iron, ferrocyanide/hydrochloric acid, water, nuclear fast red |
what is the function of the 12% acetic acid and colloidal iron in M.M. Colloidal Iron | acetic acid 12% prevents watery dilution of colloidal iron, colloidal iron stains the acidic mucosubstances |
what does the ferrocyanide/HCL do in M.M. Colloidal Iron | stain carboxylated and sulfated mucosubstances |
what does the water and nuclear fast red do in the M.M. Colloidal Iron | water washes out the excess reagent, and the nuclear fast red is the counterstain |
typical results of M.M. Colloidal Iron | acid mucosubstances and sialomucins - dk blue, nuclei - pink red, cytoplasm - pink, cryptococcus - dk blue |
what can PAS do for M.M. Colloidal Iron | can act as a counterstain (if PAS positive it will stain magenta), and acid/neutral/mixes of mucosubstances will be purple, acid mucosubstances ONLY will be Blue |
M.M. Colloidal Iron is not specific for what? | acid mucopolysaccharides |
if the background gets overstained what should be done. | repeat using fresh solutions |
what other stain will appear visually similar to colloidal iron | alcian blue stain will appear similar to colloidal iron |