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UND 363 Mayer Muci
UND 363 Mayer Mucicarmine
Question | Answer |
---|---|
what is the purpose of Mayers Mucicarmine | Demonstrate Epithelial mucin (adenocarcinoma and cryptococcus capsules) |
what is M. Mucicarmine not good for | Connective tissue Mucins |
What is the principle behind M. Muci. | Aluminum forms chelate complex w. carmine the resulting compound had a net pos. charge and attaches to the acid group of mucin. (binding of acid groups of mucin and attaching a red color to the complex in a dye lake formation) |
fixatives for M. Mucicarmine | 10% NBF |
thickness and control | 4-5microns, use colon, sm. intestine, appendix (due to goblet cells in these specs) |
microscope for M. mucicarmine | Light |
what are the three reagents in mayers mucicarmine | Weigert iron hematox = nuclear stain mayers mucicarmine = chelating agent Metanil Yellow = counterstain |
What are the expected results | Mucin - deep rose/red (goblet cells), Cryptococcus capsule - deep rose/red, nuclei = black, background = blue or yellow |
what are some precautions to be aware of | can get over-stained with metanil yellow an hematox. Over hematox (commonly caused by fresh weigerts hematox) will result in pink areas having a grey tint |
what are two best practices with mayers mucicarmine | use a control with less mucin (instead of one overloaded with it); use under hood because of anhydrous chloride (due to hcl vapors) |
what are mucins and what 4 props do they have | Mucin are intracellular secretions of various cells. 1. stain with basic dyes. 2. metachromatic (means stain a diff color than the stain itself) 3. precipitated by acetic acid (except gastric mucin). 4. soluble in alkaline solutions. |
what can tap water do in the mayer mucicarmine technique | tap water can remove metanil yellow |
what will be seen if autolyzed colon is used for a control in this stain | no goblet cells will/can be positively identified. |