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UND 363 Best Carmine
Question | Answer |
---|---|
Best Carmine Purpose | Demonstrate Glycogen (though PASD is a better option) |
Principle of B. Carmine | Hydrogen bonds form between glycogen and carminic acids (ie hydroxyl *OH* groups of glycogen form hydrogen bonds w/ hydrogen atoms of carminic acid) |
Fixatives for B. Carmine | Absolute alcohol (BEST), Carnoys, Bouins |
section thickness and control option | sections at 4-5microns, Liver for control (DO NOT USE AUTOPSY *will likely be depleted of glycogen* |
Microscope for B. Carmine | Light |
Name the 3 reagents used in B Carmine | Carmine, harris hematox, and a differentiating solution |
what is the order of the stains in B. Carmine | Harris (first) as a nuclear stain, then Carmine (second) as a normal stain, lastly put in differentiating solution for the glycogen |
what are the three components of the differentiating solution for B. Carmine | Absolute, Methanol, distilled H20 |
What results should be expected with B. Carmine | Glycogen = rose pink Nuclei = blue |
B. Carmine contains ammonia why is this significant and what can be done | ammonia is prone to evaporate and leave precipitate of dye on slide ** filter solution before using and keep in closed container** It is also a sever eye irritatnt and corrosive and should be USED under a HOOD |