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# ADV.D04, Ch.8 Eqp.
# ADV.D04, Ch.8 Tubes & Equipment
Term | Definition |
---|---|
EDTA tubes | lavender, royal blue, tan, white, pink |
Heparin tubes | light green with gel and green without gel. Both are used for STAT chemistry tests. Heparin controls potassium (K+) levels. |
Thrombin tubes | Orange or Gray/yellow. Blood clots quickly. Reduces "wait time" before testing. Used for serum chemistry tests. |
Gray tubes | Have calcium oxalate and sodium fluoride. Sodium fluoride preserves the glucose. Used for blood sugar (glucose) tests. |
SPS tubes Sodium polyanethanol sulfonate | Blood culture transport tube. Keeps most aerobes and anaerobes alive until blood can be put into culture bottles. |
Light Blue tube | Used for coagulation (blood clotting) tests. Mostly used for PT (prothrombin time) and PTT (partial thromboplastin time). |
Nonadditive tubes | Gold top is a safety top type. Red/Gray is a gel tube (SST). Red plain tube is for immunology and immunohematology. |
clot activator | glass / silicone beads sprayed inside the tube. |
thixotropic gel | separation gel used in red/gray serum tubes. Also called a polymer gel. It has a specific gravity lighter than water. It separates blood cells from the serum |
serum | the liquid part of clotted blood |
plasma | the liquid part of unclotted blood |
glycolytic inhibitor | anything that can stop glycolysis (breaking down sugar). Fluoride preparations (gray tubes) do this. |
gauge | A number that represents the width of a needle |
whole blood | Blood with both the liquid and cells together. |
See appendix 7 | Good reference for what tube to use for a lab test. |
anticoagulant | chemical additive that prevents blood from clotting. |
oxalate, citrate, EDTA, heparin | removes calcium from the clotting reactions and blood does not clot |
effect of heparin on RBCs | Alters the wright's stain for peripheral smears. Also known to distort the shape of RBC. |
aseptic | without bacterial contamination |
anaerobic bottle | designed for growing anaerobic bacteria |
anaerobic | "without oxygen". A type of bacteria that needs CO2 and not oxygen (O2). They are killed by oxygen (O2). |
aerobic | A type of bacteria that needs oxygen to live. |
blue top tube requirements | Must be full. Test cannot be done if tube is underfilled. |
length of time for blood to completely clot in a plain red top tube. | 30 minutes |
SST | serum separation tube: |
plain red top tube | used for blood bank (immunohematology) and immunology departments. This has no polymer gel and no clot activator. |
Green top tubes | Tube may have Sodium heparin (Na), Ammonium heparin (NH4), or Lithium heparin (Li) anticoagulants. Used for STAT chemistry tests. Also controls potassium level. Clotted blood contains a higher level of potassium. |
Lavender EDTA tube | Used for hematology: Red cell count, White cell count , Platelet count, RBC indices : MCH, MCHC, MCV |
How many gentle inversions (mixing) do you do for blood tubes | 8 to 10 times for most tubes. 4x for small light blue tubes |
Gray top tubes | Contains fluoride. fluoride is an antiglycolytic agent. This means it prevents the red cells from using the glucose in the blood. |
Royal blue top tubes | with or without EDTA. Used for finding trace elements and some nutritional elements that exist in small amounts in the blood. |
Tan top tubes | Has EDTA. Used for lead determinations |
what is the correct order of draw for microtainers | 1. edta 2. other additive tubes 3. non additive tubes |
what is the correct order of draw for vacuum tubes | 1. Yellow (SPS) 2. Light blue (citrate) 3. Serum tubes 4. Green tops 5. EDTA (lavender, pink, black, royal blue) 6. gray top (fluoride) |
Yellow top with black band is for... | Blood cultures and the tube has SPS. |
Yellow top for cell preservation is the | ACD tube. Acidified Citrate Dextrose. |
light blue coagulation tube has... | sodium citrate |
Plain red top tube has... | no additives |
gold safety top tube has gel and | clot activator |
The tiger top tube is also called the... | SST (serum separator tube) |
A green top with gel has... | sodium heparin and thixotropic gel |
light b lue top tube has... | sodium citrate. Tube must be filled completely or the tests cannot be done. |