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Micro-Lab001

Special media for isolation

QuestionAnswer
why don't you have to perform a Gram stain on each organism from every selective medium? The bacteria will have the same Gram reaction because only one type can grow on selective medium.
Are mannitol salt agar (MSA) and eosin methylene blue (EMB)differential or selective? Both selective
What's the selective purpose of mannitol salt agar (MSA)? Grows Gram positive bacteria
What's the selective purpose of eosin methylene blue (EMB)? Grows Gram negative bacteria
Peptone's purpose in media? It's a nutrient. Bacteria that cannot use the sugars in the media use peptone to grow.
Agar's purpose in media? It's the solidifying agent.
What ingredient makes mannital salt agar (MSA) selective? High concentration of salt aka sodium chloride aka NaCl
Name the bacteria that: appears metallic green on eosin methylene blue plate(EMB) E. coli
Name the bacteria that: appears colorless on eosin methylene blue plate(EMB) P. aeruginosa
Name the bacteria that: appears yellow on mannitol salt agar (MSA) M. luteus
Name the bacteria that: appears white on mannitol salt agar (MSA) S. epidermidis
Nutrient agar with vancomycin is selective or differential? What for? Selective. For vancomycin resistant organisms.
Minimal salts agar that contain detergent as a carbon source is selective or differential? What for? Selective. For autotroph that can survive with little nutrients other than carbon, or a heterotroph, which needs a food supply and will die without one.
Nutrient agar with tellurite (coagulase indicatior-identifies whether an organism produces the exoenzyme coagulase, which causes the fibrin of blood plasma to clot)is selective or differential? What for? Differential. Staphylococcus aureus is known to produce coagulase, not all Staph bacteria do
Created by: 100001455498513
 

 



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