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Step 1 9.21.12
Microbiology I
Question | Answer |
---|---|
What is the function an composition of peptidoglycan? | gives rigid support, protects against osmotic pressure. made up of sugarbackbone with cross linked peptide side chains |
What is the function and chemical composition of cellwall and membrane in G+? | major surface Ag made up of peptidoglycan wiht lipoteicoic acid. |
What 2 cytokines does lipoteichoic acid ilicit in the host? | TNF, Il-1 |
what is the function and chemical compostions of the outer mebrane in G-? | site of endotoxin(lippopolysacaride major Ag). polysaccaride, lipid A |
What 2 cytokines does endotoxin (lipopolysaccardie ellicit in the host? | TNF, IL-1 |
What is the function and composition of the plasma membrane in bacteria? | lipoprotein bilayer. site of oxidative and transport enzymes |
What are the subunits and function of the bacterial ribosome? | 50S and 30s, protein synthesis |
What is the function and composition of a bacterial capsule? | protects bacteria from phagocytosis, made of polysaccaride except Bacillus anthracis where it is D-glutamate |
What is the function and composition of the periplasm in bacteria? | space between cytoplasmic membrane and cell wall in G-. contains hydrolytic enzymes like beta lactamases |
What is the function and composition of the bacterial pilus/fimbria? | made of glycoprotein, mediate adherence and sex pilus allows for conjugation between 2 bacteria |
What is the function and composition of a bacterial spore form? | helps resist dehydration ,heat, chemicals. made of keratin like coat of dipiclonic acid |
Where in the bacteria do genes for abtx resistance tend to be held? | on plasmids |
What is the function and composition of a bacterial glycocalyx? | mediates adherence to surfaces, esp foreign ones(catherters). made of polysaccardie |
What is the difference between G+ and G- bacteria biochemically? | G- have 2nd cell wall made of endotoxin, G+ have lipoteichoic acid projections from cell wall |
What are 2 examples of gram positive cocci? | staphylococcus, streptococcus |
What is an example of a G- cocci? | Nisseria |
What are 5 G+ bacilli? | clostridium, corynebacteria,bacillus, listeia, acid fast mycobacteria |
What are 9 major G- bacilli? | ALL ENTERICS, haemophilus, legionella (silver), bordetella, francisella, brucells, pasteurella, bartonella, gardnerella (G variable) |
What are 2 examples of branching filamentous G+? | actinomyces, norcardia (weakly acid fast) |
What are 2 examples of pleiomorphic G-? | rickettsiae, Chlamydia (giemsa) |
What are 3 examples of G- spirochetes? | leptospira, Borellia (giemsa),treponema |
What bacteria has no cell wall? | mycoplasma |
What is unusual about the mycoplasma cell wall? | doesnt have one, contain sterols in membranes |
What is unusula about the mycobacteria cell wall? | contain mycolic acid, high lipid content |
What are 6 bacteria which do not gram stain well? | 1.Treptonema (too thin) 2. Rickettsia (intracellular) 3. Mycobacteria (high lipids require acid fast) 4. Mycoplasma (no cell wall) 5. Legionella pneumophila (mostly intracellular) 6. Chlamydia (intracelular, lacks muramic acid in cell wall) |
What is the pneumonic for bacteria that can't be stained with gram? | These(treptonema) Rascals(rickettsia) May(mycobacteria) Microsciopically (mycoplasma) Lack (legionella) Color(chylamidia) |
What is the the best way to stain legionella? | silver stain |
What is the best way to stain borellia, Plasmodium, trypanosomes, Chlamydia? | giemsa stain |
What is the use of a periodic acid schiff stain (PAS)? | stains glycogen, mucoopolsaccarides, used to Dx Whipples (tropherma whipelli) |
What is the use od Ziehl-Neelsen stain(carbol fuchsin)? | stains acid fast organismsa |
What is used to stain for cryptococcus neoformans? | india ink stain |
What stain is best for pneumocystis and legionella? | silver |
What organism has to be grown in chocolate agar with Facor V(NAD+) and X(hematin)? | H. influenzae |
What organism requires growth in Thayer-Martine (VPN) media (Vancomycin (killG+), polymixin (G-), nystatin (fungi) | N. gonorrhoeae |
What organism requires Bordet-Gengou agar? | b. pertussis |
What organism requires tellurite plate, Lofflers media? | C. diptheria |
What organism requires Lowenstein-Jensen agar for growth? | M. tuberculosis |
What organism requires Eaton's agar to grow? | M pneumonidae |
What types of bacteria are pink on MacConkey's agat? | lactose fermenting enterics |
What bacteria makes blue black colonies on EMB agar? | E. coli |
What organism requiores charcoal yeast extract agar with cysteine and iron? | legionella |
What organisms require Sabouraus agar for growth? | fungi |