Histology ASCP Word Scramble
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Question | Answer |
dehydrating agents | alcohol, acetone, dioxane, tetrahydrofuran (last two are universal solvents) |
incomplete dehydration results in | mushy soft sections |
the dye-check solution of eosin can only be added to ________, not isopropanol | ethanol (dehydratant) |
__________ cannot be used in processors or with isopropanol | Drierite (dehydratant) |
clearing agents must be miscible with ______ and _______. | dehydratant and paraffin |
a reagent that CANNOT be used for dehydrating tissues is: | benzene (clearnat) |
paraffin used for embedding is ___ degrees celsius. | 53 degrees celsius |
one disadvantage using dioxane for processing is | it is TOXIC |
one xylene disadvantage is | it is likely to harden tissue |
routine use paraffin melting point should be | 55 to 58 degrees C |
which is not a clearing agent? chloroform/ethanol/dioxane/xylene | ethanol (dehydratant) |
overheating of paraffin used for embedding may cause | cracking of the block |
one advantage of paraffin use is | serial sections are easy to obtain |
when cutting a block, if the tissue seems hard and brittle... | overheated paraffin |
SOFT paraffin is most useful when _______ sections are desired. | thick |
when used for clearing, cedar wood oil must be followed by | xylene (clearant) |
paraffin is cooled rapidly as possible after embedding to prevent | formation of large crystals |
dehydration and clearing steps can be omitted when | using water-soluble wax (embedding medium) |
ETHYL ALCOHOL | BEST DEHYDRATANT!!! PEL=1,000 / 60-65-95-95-absolute |
denatured alcohol | ethanol + methanol or ethanol + isopropanol (dehydratant) |
METHYL ALCOHOL | POISONOUS dehydratant, NOT USED ALONE PEL=200 / not for skin; breaks down to formaldehyde; causes blindness and death |
Dioxane | a UNIVERSAL SOLVENT which is faster, less shrinkage than ethanol, toxic, rarely used / PEL-OSHA=100 /PEL-NIOSH=1 |
ISOPROPANOL | a dehydratant that CANNOT BE USED IN PLACE OF ETHANOL WHEN STAINING! PEL=400 |
Acetone | (dehydratant) PEL-OSHA=1,000 / PEL-NIOSH=250 / (rapid, cheap, but absorbs water from the air) USED IN TOUCH PREPS OR FAST FIXES. CAN ALSO BE USED LIKE A UNIVERSAL SOLVENT. |
BUTYL ALCOHOL | 4 PLANTS AND ANIMAL MATERIAL! PEL=100 (dehydratant) |
Tertiary Butanol | (dehydratant)odorous, expensive, solidifies at room temperature,MIXES WITH WATER, ETHANOL, XYLENE / PEL=100 |
Tetrahydrofuran | a universal solvent that is miscible in lower alcohols, water, xylene, toluene, benzene, chloroform, acetone,melted parrafin. less toxic than dioxane. PEL=200 / offensive odor, causes conjunctivitis/ BEST UNIVERSAL SOLVENT |
PEL=100 / used as clearing agent in most labs | xylene (clearant) |
Toluene | BEST HYDROCARBON CLEARING AGENT, PEL=50, STEL=150 /not as hard as xylene |
Benzene | fast, does not over-harden, WILL HARDEN MUSCLE, TENDON, UTERUS more than TOLUENE // PEL=10 |
XYLENE SUBSTITUTES ARE | ALIPHATIC HYDROCARBONS AND LIMONENE |
CHLOROFORM PEL=50 | chloroform is carcinogenic |
water soluble waxes | CARBOWAX: dehydrating and clearing unnecessary. Infiltrates tissue directly from fixative.BAD: floating out occurs |
a nitrocellular compound used for embedding (i. Parloidion). storage blocks held in 80% alcohol. hardened with chloroform. mixed with ether and absolute alcohol to prepare. | celloidin |
great for undecalcified bone, kidney, bone marrow, lymph node biopsies | GMA Glycol Methacrylate , acrylic resin which is miscible with water. |
5% aqueous formic acid will | decalcify most bones in 3 to 5 days |
celloidin MUST BE | dehydrated |
ethylene glycol monoethyl ether | a dehydratant that retains tissues for extended periods of time. more than ethanol or 70% alcohol |
sections processed by _______ method show less shrinkage than when processed by paraffin. | celloidin |
best processing for enzyme demonstration | frozen sections |
ion exchange resin & electrolytic methods of decalcification are considered | acid methods |
these agents EDTA)bind certain metals | chelating agents |
30% sucrose | best way to get frozen sections from formalin fixed, unprocessed tissue, by infiltrating in this |
Created by:
alexinem
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