UND 363 Crystl Viole Word Scramble
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Question | Answer |
Purpose of Crystal Violet | demonstrate amyloid (not a specific as congo red) |
principle of Crystal Violet | *not clearly understood* polychromatic (ie single dye that stains components different colors) |
the addition of acid to Crystal Violet will prevent what | overstaining of the cytoplasm |
fixatives of Crystal Violet | 10% NBF or alcohol |
thickness and control | 10-12 micron, control must be a known positive for amyloid |
microscope of Crystal Violet | light |
name the 2 reagents for crystal violet | crystal violet - stains amyloid apathy mounting media |
why is apathy mount media needed | there is potential bleeding of basic aniline dyes into surrounding mouting medium when aqueous media is used |
what does apathy mount have that prevents bleeding | sugar and salt |
what can be used instead of apathy and how | synthetic resins can be used but stained sections must be completely air dried and then dipped in xylene before coverslipping |
typical results of Crystal Violet | amyloid - purplish violet other tissue - blue |
Created by:
mustangvxd
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