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Bacterial Cell StructureCapsule-no plasma membrane, flagellum-swimmers, ribosomes-dna, neucloid area-no nucleus, no membrane bound organelles, pilus helps to anchor to another surface to transfer genetic material
Bacteria growth requirementsH2O, PH (6.0 -*.0, 7.0 is optimal), Temp 0-90 C
Bacteria MediaGround Seaweed-Blood, protein, vitamin, dye, sugar-blood for strep or staph
Bacteria MorpholosyBacilli=rod shaped, Cocci= round, spirilla= corkscrew
Binary FissionBacteria split into two clones...ecoli in 20 mins, TB every few days
Cell Stainingstains react to chemical in cell wall, gram positive= blue(ampicillin-penicillin), gram negative= red (synthetics-carbenicillin, gentamicin)
Chlamydiaeintracellular parasite, requires host, spirilla, parasite of birds and mammals, airborne and direct contact, (trachoma, parrot fever, std's)
conjugationplasmid moves to another bacteria cell and shares info (dna) allowing each to have the same dna
Endosporesdormant stage of bacteria- most bacteria but not all have spores, all bacillus & clostidium have endospores-resistant to heat/cold, radiation, toxins, o2 in atmosphere, chemicals, uv light.
FungiThe great decomposer's of nature-made of chitin (sugar)-20-30 C temp range-few cause disease to man-eukariotic- mycology, the study of fungus-spread via spores.
Gram Stainused to ID morphology & antibiotics for treatment-crystal violet (blue)=gram negative...Safranin (red)=Gram positive
Human Fungal DiseasesAspergillosis-fungus ball in chest, coccidiodomycosis=valley fever, histoplasmosis Mississippi valley fever, candidiasis thrush
Hyphaestock of fungi
morphologystudy of shape and structure- strep = chains, staph = clumps, diplo = two stuck together
mycelliummass of fungi
normal floramicroorganisms that grow on the human body that help protect us
plaguebubonic-lymph, numonic-airborne
pneumoniabiggest killer in history
prokaryotescell with non membrane bound organelles, enclosed in capsule, no nucleus, flagella...ie bacteria, rickettsia, chlamydiae
rickettsiaeintracellular parasite, requires host, rod shaped-coccoid, leaky cytoplasm, parasites of arthropods and insects, passed via eggs (typhus/rmsf/Qfever/lyme disese)
sputum C&S (culture & sensitivity)lab culture that checks for antibiotic results.
strepnemoniamost common cause of pneumonia-rusty colored sputum
TB Stainingspecial staining process- very tough, bullet proof shell needs special stain, ziehl-neelsen (acid fast stain) carbonfusion-red= TB+ methylene-blue= TB-
Virus GrowthVirus reproduce inside living cells most vaccines are made inside eggs-chicken embryo's
Phillicspsychrophillic=0-25 C, Mesophillic=20-45 C, Thermophillic= 45-75 C, Extreme Thermophillic 100+ C
Capneicbacteria that like CO2
Facultativebacteria that may or may not use O2
CoccidiodomycosisValley Fever
Human Fungal DiseasesCoccidiodomycosis (valley fever), candidiasis (thrush)
Nosocomial infectioninfection acquired in the hospital
Paramyxo Virusstable dna virus, ie measles, mumps, rsv
PCP Pneumocystic Carninii Pneumonianormal flora, acts like fungus, AIDS patients die from it
ProtozoaEucaryotes, lives in or near water, unicellular, few cause disease. cause malaria, dysentery, sleeping sickness
Plasmodium speciesmalaria
RetrovirusRna virus produces the enzyme reverse transcriptase, allowing it to produce DNA, example is HIV
RSVRespiratory Syncytial Virus, causes bronchiolitis in small children
TB treatmentRIPE Rifampin, Isoniazid, Pyrazinamide, Ethamutol
VirusBad news in a protein coat, intracellular parasite, very tiny, no organelles or nucleus, contains DNA or RNA
Virus Latencyviral genetic material is incorporated into host cell but no replication takes place
Virus LysogenyLytic cycle=cell is infected and viral replication takes place
Virus Structuresurrounded by a protein capsid, fulled with DNA or RNA, spikes on capsid attach to other cells