Micro Practical 6/12
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| How do you label a Petri dish? | Name, date, time, site, Drs name
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| How do you incubate a Petri dish? | agar side up
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| Culturette- what is the purpose of transport media? | Prevent swab from drying out
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| How do you label a culturette? | Name, date, time, site, Drs name
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| What temp do you incubate at? | 37 degree Celsius
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| What is the isolation streaking technique? | 3 sections. Circle drawings. 8 swipes. 3 stabs in original quadrant
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| What is aseptic technique? | pinky finger on dominant hand is used to take off the cap of tube
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| What stains are used in Gram Stain? | Crystal violet, iodine, gram stain decolorizer, safrinin
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| What microscope objective is used to report smear? | 97x with drop of oil
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| What is SBAP? | Sheep blood agar plate
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| What is alpha? | Green halo
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| What is Beta? | clear around colony
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| What is Gamma? | no change
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| Where is the bacitracin disk placed? | placed in original area
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| What does the bacitracin disk identify? | Group A Strep
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| What equipment is used to Rapid Strep Testing? | 2 swabs, tongue depressor and test kit
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| What does the rapid strep test, test for? | Group A beta hemolytic streptococcus- strep pyogenes
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| What areas do you swab while swabbing the throat? | Tonsillar area, back of throat, include suspicious areas
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| What technique is used to swab the throat? | Figure 8 motion
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| What does Hemoccult test for? | Blood in stool
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| What areas should you get a sample of for a Hemoccult test? | black slimy areas
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| How do you know if a Hemoccult test is positive? | Turns blue
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| What is the patients prep for Hemoccult? | no red or rare meat 2 days prior or during test
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| When do you develop the control on a Hemoccult test? | After results are developed, interrpreted, and recorded
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| What equipment is used to collect a urine culture? | Uricult
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| What media is used to collect a urine culture? | CLEB, EMB
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| How do you interpret the number of organisms on Uricult? | Check against sheet. <10,000, 10,000-100,000 and >100,000
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| How do you determine the gram stain of an organism on Uricult? | CLED- gram pos/neg
EMB- Only gram neg
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| How is a wet mount slide made? | One drop of saline
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| What microscope objectives are used for Wet Mount? | focus on 10X then go to 45X
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| What organisms are seen for a Wet Mount? | Yeast
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| What is the patient prep for O & P? | No laxatives, antacids, or stool softeners for 7 days
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| How is the sample to be collected for 0 & P? | Urinate first, sample must be collected in container. Can NOT be collected from toilet
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| How much do you fill the vial for O & P? | Till red fill line
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