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Lipids, Membranes, and Membrane Transport

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Question
Answer
How do integral membrane proteins get into the membrane?   cross membrane once; cross membrane multiple times; greasy feet; bound to another membrane protein  
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How to proteins span the membrane bilayer? Solution 1   multiple amphipathic helices can associate to form a channel through the lipid bilayer.  
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How to proteins span the membrane bilayer? Solution 2   beta-Barrels proteins function as transporters  
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FRAP   Fluorescence Recovery After Photobleaching. It's how proteins diffuse in the plane of the membrane.  
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How are the proteins restricted of their mobility?   anchored to the cytoskeleton or the extracellular maxtrix; bound to another cell; enclosed by a diffusion barrier  
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What can pass through the lipid bilayer?   small hydrophobic molecules (O2, CO2, N2, Benzene); small unchared polar molecules (H2O, Glycerol, Ethanol)  
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What CAN'T pass through the lipid bilayer?   Larger polar molecules (amino acids, nucleotides, sugars); ions (H+, Na+, K+, Ca, OH-)  
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What are the two major classes of membrane transport proteins?   Channels and carriers  
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Channels   form a pore in the membrane, allowing molecules to pass through membrane and down gradient  
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Carriers   transfer molecules across membrane. Does not form an open channel.  
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What kind of "things" require carrier to transport them through the membrane?   lysosome, nucleotides, sugars, amino acids, Na+ and K+, ATP and ADP, Pyruvate  
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What does passive transport mean?   there is no energy involved, "things" goes from high to low concentration; down Gradient  
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What is active transport?   Up gradient; requires energy, from low to high concentration.  
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Where does the energy for active transport come from?   ATP, light, electrons  
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What is Na+ K+ ATPase do?   uses energy of ATP hydrolysis to pump Na+ out and K+ in  
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Explain how Na+ K+ ATPase works. Step 1-3   1) 3 Na+ bind to cyotplasmic domain 2) PO4^ -2 transferred from ATP to protein, ADP released. 3) Na+ transferred across membrane.  
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Explain how Na+ K+ ATPase works. Step 4-6   4) 2 K+ bind to external domain. 5) PO4^ -2 cleaved from protein. 6) K+ transferred across membrane  
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What is the net of the Na+ and K+ of Na+ K+ ATPase?   3 Na+ out and 2 K+ in per ATP  
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Electrogenic pump   creates a voltage across membrane.  
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Plants use (blank) for symport in place of (blank)   H+, Na+  
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How is the osmotic equilibrium of animal cells maintained?   by the Na+ K+ pump  
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What if the Na+ and K+ pump is inactivitated with oubain if if ATP is depleted??   Ion gradient collapses, and water is drawn into cells by osmosis. Cell swells and bursts  
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How does plant cells oppose osmotic swelling forces?   by cell walls  
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