Order of blood draw and color-coded tops for blood draw
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| Order of Blood Draw (First) Blood Culture Tubes: | Yellow-Sterile are first. These tubes are sterile. The phlebotomist does not want to transfer from nonsterile tube to a sterile tube.
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| Order of Blood Draw (Second) Coagulation Tests: | Light Blue Tops are second, (next). These tubes are always drawn before tubes with other kinds of anticoagulants.
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| Blue Tubes | Phlebotomist must be aware NOT to have cross contamination with other additives that could interfere with coagulation.
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| Order of Blood Draw (Third) Clot Activators: | Red and or Red/Gray (Gold) and plastic red top tubes are next. These tubes contain clot activators. AGAIN ALL EXCEPT THE RED GLASS TUBE. NO ADDS.
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| Red Glass Top Tubes: | It is safe to draw glass red top tubes because these tubes do not contain additives.
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| Order of Blood Draw (Fourth) Heparin: (Green Top Tubes) | Additive-anticoagulant sodium heparin or lithium heparin.
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| Order of Blood Draw (Fifth) (Lavender) | Lavender Top tubes The EDTA binds to calcium and may give a falsely low calcium and falsely high potassium reading. Lavendar tubes are drawn near the end of the multi-tube draw.
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| Order of Blood Draw (Last) Contains Potassium Oxalate: (Gray) | The Gray Top Tube: This tube can elevate the potassium levels measured in an Electrolyte Analysis. It can damage cel membranes
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| Sodium Fluoride: | additive to Gray Top tubes.
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| Red Glass Tube: | Test include chemistry, serology, blood bank. But mostly Theraputic drugs.
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| Red Glass Tube Additives: | None, (Specimen): Serum,
separated by centrifuge after clotting.
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| Red Plastic Tube: | Tests include chemistry, serology
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| LIght Blue Tube: | Tests include Coagulation Testing.
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| Light Blue Tube Additives: | Sodium Citrate, (Specimen) Plasma, Inversion (3-4 times).
Notes on Process: Must fill tube completely to maintain the ratio of nine parts blood to one part sodium citrate.
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| Lavender Tube | Tests include Complete blood count (CBC), WBC, platelet count.
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| Lavender Tube Additive: | EDTA (Specimen)Whole Blood, Inversion (8).
Notes on Process: None; most common blood test ordered, so lavender tops are the most frequently used.
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| Gold or Red/Gray Stopper Tube: (SST)Serum Separator Tube) | Tests include Most blood Chemistry Panels, Sodium, Potassium, electrolytes.
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| Gold or Red/Gray Tube Additive: (SST's) | Clot activators, thixotropic gel. (Specimen) Serum. Inversion (5).
Notes on Process: None; a.k.a., serum separator tubes (SST;s), star tubes, tiger top or jungles top.
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| Gray Tube: | Tests include Lactic acid measurement, glucose tolerance test (GTT), fasting blood sugar (FBS), blood alchol level.
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| Gray Tube Additives: | Iodoacetate, Sodium fluoride; may also have anticoagulant potassium oxalate or heparin.
(Specimen) Plasma. Inversion (8.
Notes: None
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| Black Tube: | Tests include sedimentation rate.
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| Black Tube Additives: | sodium citrate. (Specimen) Whole blood, Inversion number(0).
Notes on process. No inversions because everything must settle in a sedimentation study; must fill tube completely to maintain the ration of four parts blood to one part sodium citrate.
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| Green Tube: (PST) | Tests include STAT blood chemistry, ammomia, electrolytes, arterial blood gases (ABG), human leukocyte antigens (HLA), chromosome analysis.
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| Green Tube Additives: | Heparin, (Specimen), plasma, Inversion (8).
Notes: None
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| Light Green or green/gray stopper: | Tests inclue, STAT pottasium.
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| Light Green or green/gray stopper Additives: | Heparin; thixotropic gel, (Specimen), plasma.
Inversion (8)
Notes on Process: None, a.k.a, plasma separator.
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| Orange or Yellow/Gray stopper: | Tests include STAT blood chemistry.
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| Orange or Yellow/Gray Additives: | Thrombin, (Specimen): Serum, Inversion (8).
Notes on Process: allows for five minutes of clotting time for patients on anticoagulant therapy.
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| Royal Blue: | Tests include: toxicology, trace metals, nutritional analysis
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| Royal Blue stopper Additives: | Heparin, EDTA or none, (Specimen) Plasma, or Serum.
Inversion number with heparin and EDTA-8, with no additives - 0. No notes.
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| Tan Stopper: | Tests include lead analysis.
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| Tan Additive: | Heparin, (Specimen), Plasma.
Inversion (8). No notes
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| Yellow Sterile Tube: | Tests include, Blood cultures. Contains a nutrient broth or referred to as media, which encourages te growth of microorganisms.
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| Yellow Sterile Additives: Blood Cultures | Sodium polyanethol sulfonate (SPS). Inhibits Phagocytosis, Encourages growth of microrganisms.
Notes on Process: Recovers microorganisms that are causing blood infections.
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| Yellow (ACD) Acid citrate dextrose. | Tests include: Human leukocyte antigen (HLA) studies; used for paternity testing, tissue typing, DNA and human leukocyte antigen (HLA)
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| Yellow (Non-Sterile) Additives: Do Not confuse with Sterile. | Acid citrate dextrose. (Specimen) Whole Blood,
Inversion (8). No notes.
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| Pink Tube: | Tests Include Hematology, Blood Bank.
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| Pink Tube Additives: | EDTA, (Specimen) Whole Blood, Plasma.
Inversion (8) mostly used for blood bank
Notes on process: Pink tube is similar to the lavender tube, but its closure and label must meet the standard set by the American Association of Blood Bank.
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