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Order of blood draw and color-coded tops for blood draw

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Order of Blood Draw (First) Blood Culture Tubes:   Yellow-Sterile are first. These tubes are sterile. The phlebotomist does not want to transfer from nonsterile tube to a sterile tube.  
Order of Blood Draw (Second) Coagulation Tests:   Light Blue Tops are second, (next). These tubes are always drawn before tubes with other kinds of anticoagulants.  
Blue Tubes   Phlebotomist must be aware NOT to have cross contamination with other additives that could interfere with coagulation.  
Order of Blood Draw (Third) Clot Activators:   Red and or Red/Gray (Gold) and plastic red top tubes are next. These tubes contain clot activators. AGAIN ALL EXCEPT THE RED GLASS TUBE. NO ADDS.  
Red Glass Top Tubes:   It is safe to draw glass red top tubes because these tubes do not contain additives.  
Order of Blood Draw (Fourth) Heparin: (Green Top Tubes)   Additive-anticoagulant sodium heparin or lithium heparin.  
Order of Blood Draw (Fifth) (Lavender)   Lavender Top tubes The EDTA binds to calcium and may give a falsely low calcium and falsely high potassium reading. Lavendar tubes are drawn near the end of the multi-tube draw.  
Order of Blood Draw (Last) Contains Potassium Oxalate: (Gray)   The Gray Top Tube: This tube can elevate the potassium levels measured in an Electrolyte Analysis. It can damage cel membranes  
Sodium Fluoride:   additive to Gray Top tubes.  
Red Glass Tube:   Test include chemistry, serology, blood bank. But mostly Theraputic drugs.  
Red Glass Tube Additives:   None, (Specimen): Serum, separated by centrifuge after clotting.  
Red Plastic Tube:   Tests include chemistry, serology  
LIght Blue Tube:   Tests include Coagulation Testing.  
Light Blue Tube Additives:   Sodium Citrate, (Specimen) Plasma, Inversion (3-4 times). Notes on Process: Must fill tube completely to maintain the ratio of nine parts blood to one part sodium citrate.  
Lavender Tube   Tests include Complete blood count (CBC), WBC, platelet count.  
Lavender Tube Additive:   EDTA (Specimen)Whole Blood, Inversion (8). Notes on Process: None; most common blood test ordered, so lavender tops are the most frequently used.  
Gold or Red/Gray Stopper Tube: (SST)Serum Separator Tube)   Tests include Most blood Chemistry Panels, Sodium, Potassium, electrolytes.  
Gold or Red/Gray Tube Additive: (SST's)   Clot activators, thixotropic gel. (Specimen) Serum. Inversion (5). Notes on Process: None; a.k.a., serum separator tubes (SST;s), star tubes, tiger top or jungles top.  
Gray Tube:   Tests include Lactic acid measurement, glucose tolerance test (GTT), fasting blood sugar (FBS), blood alchol level.  
Gray Tube Additives:   Iodoacetate, Sodium fluoride; may also have anticoagulant potassium oxalate or heparin. (Specimen) Plasma. Inversion (8. Notes: None  
Black Tube:   Tests include sedimentation rate.  
Black Tube Additives:   sodium citrate. (Specimen) Whole blood, Inversion number(0). Notes on process. No inversions because everything must settle in a sedimentation study; must fill tube completely to maintain the ration of four parts blood to one part sodium citrate.  
Green Tube: (PST)   Tests include STAT blood chemistry, ammomia, electrolytes, arterial blood gases (ABG), human leukocyte antigens (HLA), chromosome analysis.  
Green Tube Additives:   Heparin, (Specimen), plasma, Inversion (8). Notes: None  
Light Green or green/gray stopper:   Tests inclue, STAT pottasium.  
Light Green or green/gray stopper Additives:   Heparin; thixotropic gel, (Specimen), plasma. Inversion (8) Notes on Process: None, a.k.a, plasma separator.  
Orange or Yellow/Gray stopper:   Tests include STAT blood chemistry.  
Orange or Yellow/Gray Additives:   Thrombin, (Specimen): Serum, Inversion (8). Notes on Process: allows for five minutes of clotting time for patients on anticoagulant therapy.  
Royal Blue:   Tests include: toxicology, trace metals, nutritional analysis  
Royal Blue stopper Additives:   Heparin, EDTA or none, (Specimen) Plasma, or Serum. Inversion number with heparin and EDTA-8, with no additives - 0. No notes.  
Tan Stopper:   Tests include lead analysis.  
Tan Additive:   Heparin, (Specimen), Plasma. Inversion (8). No notes  
Yellow Sterile Tube:   Tests include, Blood cultures. Contains a nutrient broth or referred to as media, which encourages te growth of microorganisms.  
Yellow Sterile Additives: Blood Cultures   Sodium polyanethol sulfonate (SPS). Inhibits Phagocytosis, Encourages growth of microrganisms. Notes on Process: Recovers microorganisms that are causing blood infections.  
Yellow (ACD) Acid citrate dextrose.   Tests include: Human leukocyte antigen (HLA) studies; used for paternity testing, tissue typing, DNA and human leukocyte antigen (HLA)  
Yellow (Non-Sterile) Additives: Do Not confuse with Sterile.   Acid citrate dextrose. (Specimen) Whole Blood, Inversion (8). No notes.  
Pink Tube:   Tests Include Hematology, Blood Bank.  
Pink Tube Additives:   EDTA, (Specimen) Whole Blood, Plasma. Inversion (8) mostly used for blood bank Notes on process: Pink tube is similar to the lavender tube, but its closure and label must meet the standard set by the American Association of Blood Bank.  


   




 
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Created by: drehmon2237 on 2011-03-14



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