Ch 11 Stains (6)
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| What are the staining results for the Rhodanine Stain? | Copper is bright red to red yellow.(rust red) Nuclei is light blue
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| What are the staining results for the Grimelius Argyrophil Stain? | Argentaffin granules and Argyrophil granules are dark brown to black. Nuclei is red. Background is pale yellow to brown.
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| What are the staining results for the Fontana Masson Stain? | Melanin is black. Argentaffin granules are black. Nuclei is pink.
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| What are the staining results for the von Kossa Stain? | Calcium salts are black. Background is red.
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| What are the staining results for the Bile Stain? | Bile or Bilirubin is emerald green to olive . Background is yellow.
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| What are the staining results for the Prussian Blue Stain? | Hemosiderin (iron) is blue. Nuclei and hemofuchsin is bright red. Background is pink.
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| What is the control used in the Rhodanine stain? | A section containing copper.
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| What is the control used in the Grimelius argyrophil stain? | An argyrophil positive carcinoid tumor is preferred but a section of small intestine may be used.
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| What is the control used in the Fontana Masson stain? | A section of skin for melanin. A section of small intestine or appendix for argentaffin granules.
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| What is the control used in the von Kossa stain? | A section containing calcium.
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| What is the control used in the Bile stain? | A section containing bile.
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| What is the control used in the Prussian Blue stain? | A section containing ferric iron.
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| This method of staining detects ferric ions in loosely bound protein complexes (hemosiderin)? | Prussian Blue for Ferric Iron
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| This stain is cut at 6-8 microns? | Rhodanine for Copper.
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| What is Rhodanine for copper cut at? | 6-8 microns.
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| What chemical reagents that causes a reaction with iron, are sections treated with using the Prussian Blue stain? | Potassium Ferocyanide.
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| Using this stain,sections are treated with an acidic solution of potassium ferocyanide? | Prussian Blue.
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| What reagent causes an insoluble bright blue pigment in the Prussian Blue stain? | Potassium Ferocyanide.
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| Abnormally large deposits of what, may be seen in hemochromatosis and hemosiderosis? | Iron.
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| Where in the body is ferric iron normally found in small amounts? | Bone marrow and spleen.
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| Which stain are alcohols preferred as a fixative for? | von Kossa for calcium
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| Which two stains can be fixed with alcohol as well as NBF? | Prussian Blue and von Kossa.
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| Which stain CANNOT be fixed with alcohol and why? | Fontana Masson because alcohol dissolves argentaffin granules.
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| All six stains CAN be fixed in NBF, true or false? | True
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| What does the Prussian Blue stain detect? | Ferric Iron.
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| What does the Fontana Masson stain detect? | Argentaffin substances. (melanin, argentaffin granules of carcinoid tumors and neurosecretory granules)
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| What does the Grimelius Argyrophil stain detect? | Argyrophil granules.
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| What does the Bile stain detect? | Bilirubin.
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| What does the von Kossa stain detect? | Calcium.
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| What does the Rhodanine stain detect? | Copper.
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| The purpose of this stain is for the detection of copper, especially the liver, in Wilson's disease? | Rhodanine Method for copper.
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| This stain identifies calcium? | von Kossa.
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| This stain identifies bilirubin? | Bile stain.
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| This stain identifies argyrophil granules in neurosecretory tumors? | Grimelius Argyrophil
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| This stain demonstrates argentaffin substances? | Fontana Masson
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| Name the three argentaffin substances? | Melanin, argetaffin granules of carcinoid tumors, and neuro-secretory granules.
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| This stain detects ferric iron? | Prussian Blue.
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| iron that is strongly bound, as in hemoglobin, will or willnot react to the Prussian Blue stain? | It will not react.
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| A specific and easily identifiable green color develops when bilirubin is oxidized in what? | Biliverdin in an acid medium.
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| What happens during the Bile stain when bilirubin is oxidized to biliverdinin an acid medium? | A specific and easily identifiable green color develops.
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| The oxidation reaction in the Bile stain is rapidly accomplished by what? | Ferric Chloride in Tri-Chlor-acetic acid.
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| Although the reaction involved is a chemical one, it is an indirect way of decting calcium belongs to what staining principle? | von Kossa.
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| In the von Kossa stain, what two anions of calcium salts does the silver react with primarily? | Carbonate and Phosphate
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| With the von Kossa stain, BLANK reduces the silver salt to BLANK? | BRIGHT LIGHT reduces the silver salt to METALLIC SILVER.
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| In the von Kossa stain, what is sodium thiosulfate used for? | To remove unreduced silver.
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| In the von Kossa stain, what is used to tremove unreduced silver? | Sodium Thiosulfate.
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| A reaction in which certain tissue components have the ability to bind or be impregnated with silver ions and then reduce the silver to its visable metallic form is the definition of what? What stain is this associated with? | Argentaffin. Fontana Masson.
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| What stain does not need a separate reducing agent due to argentaffin? | Fontana Masson.
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| Which reaction REQUIRES a reducing agent in order to see silver in its metallic form, Argentaffin or Argyrophilic? | Argyrophilic.
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| Fontana Masson demonstrates argentaffin substances while BLANK demonstrates argyrophil substances? | Grimelius Argyrophil.
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| Certain tissue elements have the ability to bind silver ions from solutions but NO inherent ability to reduce the silver to its visable metallic form is the principle of WHAT stain? | Grimelius Argyrophil.
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| Tissue components that require an external or chemical reducer are referred to as? | Argyrophilic.
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| This stain is considered more sensitive than the rubeanic acid method for copper? | Rhodanine.
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| This stain demonstrates the PROTEIN to which the copper binds rather than the COPPER itself!? | Rhodanine
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| This staining method maybe a less specific one , giving false positive results. | Rhodanine.
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| A brown pigment that is derived from hemoglobin is the definition of what? | Hemofuscin
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| A loose complex of ferric iron and protein; Also is the storage form of iron. it is a yellowish brown pigment and is stored primarily in bone marrow. This is the definition of what? | Hemosiderin
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| An inherited disease in which there is too much copper in the body's tissues. The excess copper damages the liver and nervous system? | Wilson's disease.
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